秦皮总香豆素对实验性痛风性关节炎及尿酸代谢的影响  

Effect of Total Coumarins of Cortex Fraxini on Acute Gouty Arthritis and Uric Acid Metabolism

作　　者：赵军宁[1] 邓治文[1] 戴瑛[1] 宋军[1] 彭晓华[1] 王晓东[1] 杨安东[1] 

机构地区：[1]四川省中医药科学院药理毒理研究所,国家中医药管理局中药质量生物学评价重点研究室,中药品质评价与创新中药研究四川省重点实验室,成都610041

出　　处：《中国药学杂志》2009年第10期751-754,共4页Chinese Pharmaceutical Journal

基　　金：四川省重点科技攻关项目(99B202)

摘　　要：目的观察秦皮总香豆素对实验性痛风性关节炎及尿酸代谢的影响。方法用微晶型尿酸钠诱导大鼠足跖肿胀和家兔急性关节炎模型,观察灌胃给予秦皮总香豆素对足跖肿胀程度、关节液及滑膜组织的影响。用正常小鼠以及腹腔注射黄嘌呤造成的高血尿酸模型小鼠,观察灌胃给予秦皮总香豆素对血尿酸代谢的影响。结果微晶型尿酸钠(MSU)混悬液局部注射诱发大鼠急性足爪肿胀1～8 h,秦皮总香豆素40,80,160,320 mg·kg^(-1)剂量组的肿胀率与对照组肿胀率经统计学处理具有差异显著性。秦皮总香豆素20,40,80,160 mg·kg^(-1)剂量组可以显著抑制微晶型尿酸钠混悬液局部注射诱发急性关节肿胀和减少关节腔积液量,关节囊组织病理学观察可见,模型组滑膜组织表层可见明显充血水肿,滑膜细胞可见变性、甚至坏死,伴有淋巴细胞、中性白细胞浸润,秦皮总香豆素所试剂量范围可以显著减轻上述病理改变。秦皮总香豆素对正常小鼠血尿酸以及高尿酸小鼠血尿酸均有显著降低作用。结论秦皮总香豆素对微晶型尿酸钠混悬液局部注射诱发的大鼠足爪肿胀以及家兔急性痛风性关节炎均有对抗作用,降低正常小鼠血尿酸以及高尿酸小鼠血尿酸。OBJECTIVE To investigate the effects of total coumarins of Cortex Fraxini （TCCF） on acute gouty arthritis and uric acid metabolism. METHODS Effects of TCCF orally on edema degree of hind paw was investigated in rats with edema of hind paw induced by injection of sodium urate. Effects of TCCF orally on edema degree of joint, joint liquid, joint synovial histomorphology were investigated in rabbits with acute arthritis of hind knee joint induced by injection of sodium urate. Effects of TCCF orally on serum uric acid were investigated in normal mice and hyperuricemia mice induced by hypoxan-thine. RESULTS TCCF at dose of 40, 80, 160 and 320 mg·kg^-1 significantly reduced edema degree of hind paw by sodium urate in rats. Also TCCF at dose of 20, 40, 80 and 160 mg·kg^-1 remarkably decreased edema degree of knee joint, knee joint liquid and mended knee joint synovial histomorphology in rabbits with acute arthritis by sodium urate. Moreover TCCF at dose of 50, 100, 200 and 400 mg·kg^-1significantly lowered the uric acid in normal mice and hyperuricemia mice induced by hypoxan-thine. CONCLUSION TCCF shows a remarkable improvement effect on acute gouty arthritis and promotes the elimination of uric acid.

关 键 词：秦皮总香豆素 痛风性关节炎 尿酸代谢 

分 类 号：R965[医药卫生—药理学]