灵芝中6种主要三萜酸类成分的HPLC定量分析  

Simultaneous Determination of Six Triterpenoid Acids in Ganoderma by RP-HPLC

作　　者：丁平[1] 梁英娇[1] 罗进辉[1] 苏健芬[1] 

机构地区：[1]广州中医药大学中药学院,广州510405

出　　处：《中国药学杂志》2009年第11期822-824,共3页Chinese Pharmaceutical Journal

基　　金：广东省科技计划项目(2006B350605012)

摘　　要：目的建立应用RP-HPLC同时测定灵芝中6种主要活性成分灵芝酸A、灵芝酸B、灵芝酸C2、灵芝酸G、灵芝酸E、赤芝酸A含量的方法。方法采用Diamonsil-C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈和0.8%醋酸水溶液,梯度洗脱,流速1mL·min-1,检测波长254nm,柱温为室温。结果灵芝酸A、灵芝酸B、灵芝酸C2、灵芝酸G、灵芝酸E、赤芝酸A的质量分别在0.650～7.800,0.175～2.100,0.206～2.475,0.188～2.250,0.275～3.300,0.120～1.440μg的对数值与相应峰面积的对数值呈良好线性关系。平均回收率(n=6)分别为100.06%(RSD=2.6%),99.55%(RSD=1.6%),100.75%(RSD=1.5%),98.86%(RSD=1.9%),99.26%(RSD=2.3%)和98.95%(RSD=1.3%)。结论本方法简单、快速、准确可靠,可作为灵芝药材的质量控制方法。OBJECTIVE To establish a RP-HPLC method for simultaneous determination of six kinds of triterpenoid acids in Ganoderma, i.e. ganoderic acid A, ganoderic acid B, ganoderic acid C2, ganoderic acid G, ganoderic acid E and lucidenic acid A. METHODS The separation was performed on a Diamonsil- C18 column （4.6 mm× 250mm, 5 μm）, using a gradient elution with acetonitrile-water（containing 0.8% acetic acid）as the mobile phase.The flow rate was 1 mL·min^-1, the detection wavelength was at 254 nm and the temperature of colunm was at room temperature. RESULTS The linear relationship were obtained within the range of 0.650-7.800, 0.175-2.100, 0.206-2.475, 0.188-2.250, 0.275-3.300, 0.120-1.440 ixg for the six components respectively. The average recoveries of the six components （n=6） were 100.06%（RSD=2.6%）, 99.55 %（RSD=1.6 %）, 100.75% （RSD=1.5 %） , 98.86 % （RSD=1.9 %） , 99.26% （RSD=2.3%） and 98.95% （RSD=1.3%） . CONCLUSION The simple, rapid and reliable method could be used for the quality control of Ganoderma.

关 键 词：灵芝 三萜酸 反相高效液相色谱法 质量控制 

分 类 号：R284[医药卫生—中药学]