采用DNA和蛋白质吸收带的Kubelka-Munk光谱函数检测人结肠腺癌  

Colon Adenoma Detection Using Kubelka-Munk Spectral Function of DNA and Protein Bands

作　　者：魏华江[1] 郭周义[1] 谢树森[2] 何博华[3] 李力波[3] 陈雪梅[4] 巫国勇[5] 鲁建军[5] 

机构地区：[1]华南师范大学激光生命科学研究所、暨激光生命科学教育部重点实验室,广东广州510631 [2]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007 [3]广东药学院第一附属医院胃肠外科,广东广州510224 [4]中山大学第一附属医院眼科,广东广州510080 [5]中山大学第一附属医院心胸外科,广东广州510080

出　　处：《光谱学与光谱分析》2009年第6期1473-1477,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60778047);广东省自然科学基金项目(060250800632270);光子技术福建省重点实验室项目(FP0403);医学光电科学与技术教育部重点实验室项目(JYG0503);广东省医学科学基金项目(A2006309)资助

摘　　要：采用DNA和蛋白质吸收带的Kubelka-Munk光谱函数对人结肠腺癌进行了鉴别诊断,实验采用带积分球附件的分光光度计获取组织的漫反射光谱。结果表明:在250～650 nm,结肠上皮组织的癌变导致其上皮组织在DNA吸收带的Kubelka-Munk光谱函数f(r∞)及其对数log[f(r∞)]的平均值在260 nm处都有非常显著性的差异,其差异分别为218%(p<0.05)和68.5%(p<0.05)。结肠上皮组织的癌变导致其上皮组织在蛋白质吸收带的Kubelka-Munk光谱函数f(r∞)及其对数log[f(r∞)]的平均值在280 nm处也都有非常显著性的差异,其差异分别为208%(p<0.05)和59.0%(p<0.05)。结肠上皮组织的癌变导致其上皮组织在β-胡罗卜素吸收带(480 nm处)的Kubelka-Munk光谱函数f(r∞)及其对数log[f(r∞)]的平均值在480 nm处也都有非常显著性的差异,其差异分别为41.7%(p<0.05)和32.9%(p<0.05)。可见,结肠上皮组织的癌变导致其上皮组织中的DNA、蛋白质和β-胡罗卜素的含量都发生了非常显著的变化。这结论为快速、低成本、非入侵的结肠腺癌的光活检提供一些有益的参考。Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were Significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞）] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% （p〈0.05） and 68. 5% （p〈0. 05） respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞ ）] of the protein absorption bands at 280 nm between normal and adenomatous colon epithelial tissues, and the differences were 208% （p〈0. 05） and 59.0 % （p〈 0. 05） respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞ ）] of the β-carotene absorption bands at 480 nm between normal and adenomatous colon epithelial tissues, and the differences were 41.7% （p〈0.05） and 32. 9% （p〈0.05） respectively. Obviously, pathological changes of colon epithelial tissues were induced so that there were significant changes in the contents of the DNA, protein and β-carotene of colon epithelial tissues. The conclusion can be applied to rapid, low-cost and noninvasive optical biopsy of colon adenoma, and provides a useful reference.

关 键 词：结肠腺癌诊断 Kubelka-Munk光谱函数 漫反射光谱 DNA 蛋白质 β-胡罗卜素 

分 类 号：R318.5[医药卫生—生物医学工程]