红外光谱法对双黄连粉针剂的定性定量分析  

Qualitative and Quantitative Analysis of Shuanghuanglian Fenzhenji by FTIR

作　　者：阎姝[1] 徐茂玲[1] 图雅[2] 李惠芬[3] 孙素琴[2] 

机构地区：[1]天津中医药大学,天津300193 [2]生命有机磷化学及化学生物学教育部重点实验室,清华大学化学系,北京100084 [3]天津医科大学,天津300070

出　　处：《光谱学与光谱分析》2009年第6期1558-1561,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金地区科学基金项目(30860391)资助

摘　　要：通过分析双黄连粉针剂的红外光谱并与对照品进行比较,初步指认了粉针剂中各类成分的吸收峰,为定性质量分析提供了依据。建立了基于中红外光谱的黄芩苷与绿原酸含量校正模型。模型的R2值均在0.99以上,平均预测相对误差也低于4%,具有良好的拟合及预测能力。本研究结果说明,使用红外光谱法对双黄连粉针剂进行在线定性定量分析,方便快速,切实可行。Qualitative and quantitative analysis of Shuanghuanglian fenzhenji was studied in the present paper by FTIR. The fingerprint characteristics of main components in the injections were assigned and can be used as the criterion in the qualitative quality control. Calibration models by PLS algorithm were built to get the quantitative information of baicalin and chlorogenic acid in the injection samples. The R^2 of calibration models are both above 0.99 and the average relative deviations between predicted and actual value measured by HPLC are less than 4% for the both properties. The qualitative and quantitative analysis by FTIR is simple and rapid, so it may provide a new on-line approach to the quality control of main constituent in Shuanghuanglian fcnzhenji and some other traditional Chinese medicines.

关 键 词：双黄连粉针剂 傅里叶变换红外光谱法 定性定量分析 

分 类 号：O657.3[理学—分析化学]