心脏组织的自体荧光研究  

Research on the Autofluorescence Spectroscopy of Heart Tissues

作　　者：徐正红[1,2] 张镇西[1] 王晶[1] 李政[1] 刘雪良[1] 

机构地区：[1]电力设备电气绝缘国家重点实验室,西安交通大学电气工程学院,陕西西安710049 [2]生物医学信息工程教育部重点实验室,西安交通大学生命科学与技术学院,陕西西安710049

出　　处：《光谱学与光谱分析》2009年第6期1651-1655,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60378018,60578026)资助

摘　　要：测量并研究了家兔心脏组织自体荧光的三维光谱和发射光谱。果表明:冰冻前后心室和心房组织的三维荧光光谱差异较主动脉明显,说明冰冻后其NADH和黄素的含量改变。心房、心室和主动脉组织在340 nm激发光下的自体荧光光谱具有特定的差异,其主要荧光峰位于460 nm的NADH,以及290～400 nm之间的胶原蛋白和弹性蛋白。根据心房和心室组织的高斯拟和光谱发现,光谱的峰位、相对荧光强度和半宽等参数各不相同。由此,可以根据荧光光谱的谱形和峰强比可以显著区分出不同的心脏组织类型。此外,研究首次发现心室组织NADH的荧光强度会随心肌死亡时间增加而衰减,可作为死亡时间确定的新方法。The present study investigated the three-dimensional spectra and emission spectra of the autofluoreseence of rabbit hearts. The results suggested that the three-dimensional spectra of the iced atria and ventricle were observed more evidently different from that of the fresh tissue compared to the main artery, which indicated that the amount of flavins and NADHs changed. Also, the atria, ventricle and main artery have different specific excitation spectra at the wavelength of 340 nm. The main fluorescence peaks were of NADH （at about 460 nm）, collagen and elastin （at about 290-400 nm）. The Gauss spectra of atria and ventricle were different in the peak value, relative intensity and half width. So the ratios of fluorescence intensities of peaks may be used to distinguish different heart tissues. Furthermore, a phenomenon was firstly uncovered that the autofluorescence intensity of NADH in ventricle decays with the time of death and it could be a useful method for the estimation of postmortem interval.

关 键 词：自体荧光 荧光光谱 内源性荧光物质 心脏 

分 类 号：O433[机械工程—光学工程]