多胺代谢相关基因在小鼠子宫内膜细胞中的调节  

Regulation of polyamine-related genes in mice endometrial cell

作　　者：于永生[1,2] 罗晓彤[1] 金海国[1] 赵越超[2] 

机构地区：[1]吉林农业科学院畜牧分院,吉林公主岭136100 [2]东北农业大学生命科学院,哈尔滨150030

出　　处：《中国农业大学学报》2009年第3期17-21,共5页Journal of China Agricultural University

基　　金：国家“973”计划项目(2006CB504005)

摘　　要：利用鸟氨酸脱羧酶(ODC)siRNA干涉、ODC过表达、添加特异性ODC抑制剂二氟甲基鸟氨酸(DFMO)处理小鼠子宫内膜上皮细胞和基质细胞,采用实时定量PCR对多胺代谢相关酶类mRNA水平进行检测。结果显示:在子宫内膜上皮和基质细胞中,ODC siRNA转染后可导致SSAT mRNA水平下降为对照组的71.78%和54.36%(P<0.01),SAMDC mRNA水平上升为对照组的1.666 6倍和3.036 6倍(P<0.01);ODC过表达造成SSAT水平为对照组的1.921 4和2.852 7倍(P<0.01),AZⅠmRNA水平为对照组的1.962 1和3.073 8倍(P<0.01);利用DFMO处理体外分离的上皮细胞和基质细胞,SSAT mRNA水平为对照组的1.626 7和1.773 3倍(P<0.01),SAMDC mRNA水平为对照组的1.630 0和1.730 0倍(P<0.01),AZⅠmRNA水平为对照组的71.00%和76.67%(P<0.01);DFMO处理后上皮细胞和基质细胞数目(31.00±3.00和43.67±7.51)和活力(0.26±0.03和0.36±0.05)都显著下降(P<0.01),处于静止期的比例增加(56.33%和58.75%)高于对照组(43.46%和47.83%),处于分裂期的百分比(27.27%和27.37%)低于对照组(37.76%和40.30%)。结论:对多胺限速酶mRNA水平和酶活性的调节可引起多胺相关酶类mRNA水平的变化,维持了细胞内多胺水平的稳定,从而调节细胞的增殖。The aim of this study was to regulate the polyamine-related genes transcription in mouse endometrial cell by ornithine decarboxylase （ODC） siRNA, ODC over-expression vector and ODC specific inhibitor-difluoromethylornithine （DFMO） ,and polyamine-related genes were detected by real-time PCR . Results showed that ODC siRNA led to an obvious increase of S-adenosylmethionine decarboxylase （SAMDC） （ 1. 666 6 times and 3. 036 6 times compared with control） and decrease of spermidine/spermine N1-acetyltransferase （SSAT） transcription （71.28% and 54. 26% compared with control） in the epithelial and stromal cells, respectively . ODC overexpression caused an increase of SSAT（1. 921 4 and 2.852 7 times compared with control ） and antizyme Ⅰ （AZ Ⅰ ） （1. 962 1 and 3. 073 8 times compared with control） transcription in the epithelial and stromal cells, respectively . After mouse endometrial cell were treated by DFMO, the mRNA level of SSAT and SAMDC significantly increased（ 1. 626 7 and 1. 773 3 times, 1. 630 0 and 1. 730 0 times compared with control） and AZ I significantly decreased（71.00% and 76. 67% compared with control） in the epithelial and stromal cells, respectively. The epithelial and stromal cell viability were significant decreased and more cell maintained in stationary phase after treated by DFMO. Regulation of polyamine-related gene and inhibition polyamine synthesis could lead to the changes of polyamine-related gene mRNA, which might result in polyamine in a steady-level to regulate cell proliferation.

关 键 词：小鼠 子宫内膜细胞 鸟氨酸脱羧酶 二氟甲基鸟氨酸 多胺代谢相关基因 

分 类 号：Q954.4[生物学—动物学]