26种中草药对奶牛隐性乳房炎病原菌体外抗菌作用的研究  

Antibacterial effects of 26 Chinese herbal medicines on pathogens of dairy cow with subclinical mastitis in vitro

作　　者：刘澜[1] 周德刚[2] 莫云[1] 黄建茹[1] 葛冰[1] 臧丽 丛日刚[1] 

机构地区：[1]北京大北农集团动物保健技术研究中心,北京101407 [2]北京市兽药监察所,北京100107 [3]北京市延庆县畜牧兽医总站,北京102100

出　　处：《中国农业大学学报》2009年第3期83-88,共6页Journal of China Agricultural University

基　　金：北京市海淀区科技项目(k2008197)

摘　　要：研究26味中草药对隐性乳房炎分离得金黄色葡萄球菌和大肠杆菌的体外抗菌效果。杯碟法试验表明,黄连、黄芩、紫花地丁、板蓝根等对2种病原菌的综合抑菌效果较好,达到中敏或高敏,有的甚至是极敏,抑菌圈直径为19.00～24.93 mm。微量稀释法试验表明,黄连、黄芩、红花、金银花、板蓝根等对2种病原菌的综合效果好,MIC为0.004～0.063 g/mL,MBC为0.004～0.063 g/mL。本试验可为研究外用治疗奶牛乳房炎中草药制剂的开发提供科学依据。The in vitro antibiotic tests of 26 Chinese herbal medicines, such as Rhizoma coptidis, Radix scutellariae, Herba taraxaci and Herba violae, etc. were conducted on the isolated Staphylococcus aureus and Escherichia coli from the dairy cow with subclinical mastitis by cylinder plate and microdilution methods. The result of the plate method test indicated that the Rhizoma coptidis, Radix scutellariae, Herba violae, Radix isatidis etc. were more effective compared with other Chinese herbat medicines on the S. aureus and E. coli, and their sensitivity to the 2 pathogens are moderate or highly, or extremely high, the range of antibacterial diameters was 19. 00- 24.93 mm. The result of the microdilution method test also showed that the Rhizoma coptidis, Radix scutellariae, Flos carthami, Flos Ionicerae, Radix isatidis, etc. were more effective compared with other Chinese herbal medicines on the S. aureus and E. coil, their MIC was 0. 004 -0. 063 g/mL, and their MBC was 0. 004 - 0. 063 g/mL respectively. This study provides a scientific basis for the development of traditional Chinese medicines on the external treatments of the mastitis in dairy cow.

关 键 词：中草药 隐性乳房炎 奶牛 病原菌 抗菌试验 

分 类 号：S858.23[农业科学—临床兽医学]