延肾1号冲剂对肾缺血再灌注大鼠肾组织肿瘤坏死因子和细胞间黏附因子的影响  

Influence of Yanshen Granules 1 on kidney TNF-α and ICAM-1 in rats with renal ischemia and reperfusion

作　　者：檀金川[1] 田力铭[1] 

机构地区：[1]河北医科大学中医院肾内科,河北050011

出　　处：《北京中医药大学学报》2009年第5期321-323,335,I0002,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省科学技术研究与发展计划项目(No.04276101D-66)

摘　　要：目的探讨延肾1号冲剂抗肾缺血再灌注损伤的作用机理。方法将72只大鼠随机分为假手术组、对照组、治疗组,各24只。治疗组给予延肾1号冲剂,假手术组及对照组灌胃相应量的自来水。检测大鼠肾缺血1h,再灌注24、48h后肾功能(BUN、Cr)的变化及肾组织肿瘤坏死因子(TNF-α)和细胞间黏附因子(ICAM-1)的变化情况。结果假手术组大鼠肾小管上皮细胞内可见微量TNF-α、ICAM-1表达。与假手术组相比,治疗组、对照组TNF-α、ICAM-1分子表达增强,在肾缺血再灌注24、48h有显著差异(P<0.01);与对照组比较,治疗组TNF-α、ICAM-1分子表达下调,肾缺血1h无显著差异(P>0.05),再灌注24、48h有显著差异(P<0.01)。结论延肾1号冲剂能够保护肾缺血再灌注大鼠的肾功能并抑制其肾组织中TNF-α、ICAM-1的蛋白表达。Objective To discuss the mechanism of Yanshen Granules 1 in antagonism of renal ischemia and reperfusion injury. Method The rats （ n = 72 ） were randomly divided into the sham-operation group, control group and treatment group （each n = 24 ）. The treatment group was given Yanshen Granules 1, and the sham-operation group and control group were given same quantity of tap water. The changes of renal function indexes （BUN an Cr）, tumor necrosis factor-α （TNF-α） and intercellular adhesion molecules-1 （ICAM-1） were detected after renal ischemia for 1 hour, and after reperfusion for 24 and 48 hours. Result In the sham-operation group there were slight expressions of TNF-α and ICAM-1 observed in renal tubular epithelial cells, while in the treatment group and control group the expressions of TNF-α and ICAM-1 increased and had significant difference after renal ischemia and reperfusion for 24 and 48 hours （ P 〈 0.01 ）. The expressions of TNF-α and ICAM-1 decreased in the treatment group compared with those in the control group, and had no significant difference after renal isehemia for 1 hour （ P 〉 0.05 ） but had significant difference after reperfusion for 24 and 48 hours （ P 〈 0.01 ）. Conclusion Yanshen Granules 1 can protect renal function and inhibit the expressions of TNF-α and ICAM-1 in rats with renal ischemia and reperfusion injury.

关 键 词：延肾1号冲剂 肾缺血再灌注损伤 肾功能 肿瘤坏死因子 细胞间黏附因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]