基于FTIR反射光谱-化学计量学方法的三种车前草分析研究  

Studies on the Herba Plantaginis of Different Kinds and Habitats by FTIR Reflection Spectroscopy-Chemometrics Analysis

作　　者：孙贡磊[1] 余鹏[1] 阮琴[1] 李丹婷[1,2] 程存归[1] 

机构地区：[1]浙江省固体表面反应化学重点实验室,浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江余姚高风中学,浙江余姚315400

出　　处：《光谱学与光谱分析》2009年第7期1822-1825,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30800705);浙江省自然科学基金项目(301468)资助

摘　　要：利用傅里叶变换红外光谱(FTIRS)法测定了同科同属不同种及不同产地的车前、平车前及北美车前草的FTIR,筛选出35个比较典型的吸收峰后结合化学计量学方法进行了亲缘关系的分析研究。系统聚类分析结果表明,在距离系数约为0.036的水平上三种车前草可划分为2个类群,其中,平车前在0.033的距离水平上首先与车前聚在一起,随即约在0.042的距离水平上与北美车前草一大组,该聚类结果与依据形态特征的传统分类学结果相一致。而对不同产地车前的FTIRS主成分分析结果表明,相同生态环境下采集的车前FTIR三维散点图中距离较为相近;而地域相差比较远的样品,在三维散点图上则显示较为分散。研究方法直观、科学,简单方便,具有很好的理论与应用价值。In the present paper, FTIR of different species obtained from different regions of sibling plantaginis were determined by Fourier transform infrared reflection spectroscopy （FTIRS）, and thirty-five comparatively typical absorption peaks were selected and used to study genetic relationship, combined with chemometric methods. The phylogenetic cluster analysis revealed that three species could be divided into two groups based on the distance of 0. 036, among which Plantago depressa Willd. was clustered with Plantago asiatica L. based on distance of 0. 033, while clustered with Plantago virginica L. based on distance of 0. 042, and the result was consistent with that of traditional taxology. The principal component analysis result revealed that the distances of Plantago asiatica L. in the similar environment are similar in three-dimensional FTIR chart, however, it is dispersive when obtained from different regions. This method is scientific, simple and direct, and has important theoretical and practical application value.

关 键 词：车前 平车前 北美车前草 傅里叶变换红外反射光谱 化学计量学方法 

分 类 号：O657.3[理学—分析化学]