人肝癌组织细胞的激光光镊拉曼光谱研究  

Laser Tweezers Raman Spectroscopy Analysis of Liver Cancer Tissue

作　　者：王雁军[1] 姚辉璐 王桂文[2] 汪蕴[1] 丰美福[1] 

机构地区：[1]中国科学院动物研究所,生物膜与膜生物工程国家重点实验室,北京100101 [2]广西科学院生物物理实验室,广西南宁530003

出　　处：《光谱学与光谱分析》2009年第7期1881-1883,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30470427/C01050301);中国科学院创新重要方向性资助项目(KSCX2-SW-322)资助

摘　　要：激光光镊与拉曼光谱相结合形成的激光光镊拉曼光谱系统(LTRS)已用于分析生物组织标本,可对单个活细胞进行操控和光谱收集。从拉曼光谱特征峰位置、强度和线宽可得到有关细胞的组成、结构及细胞内物质相互作用的信息。文章应用LTRS系统,分析了来自人的恶性肝癌组织的不同病变部位标本,包括肝癌组织细胞、肝癌癌旁细胞和远离肝癌组织的肝脏正常的组织细胞,观察到了随肝癌的病变部位变化所出现的一些有趣的拉曼光谱峰的变化。正常的肝组织细胞在1 070和1 266 cm-1处的峰很明显,而肝癌和肝癌癌旁组织细胞的这两个峰则不明显,肝正常组织细胞的1 445 cm-1峰明显高于肝癌和肝癌癌旁组织细胞。已知1 070 cm-1峰代表脂类和核酸,1 266和1 445 cm-1峰代表脂类和蛋白。引起这些峰变化的物质很可能参与了肝癌的发生。上述初步研究结果表明:单细胞激光光镊拉曼光谱可以区分肝癌的不同病变部位,将是检测和分析肝癌组织标本的一种很好的方法。Single cell laser tweezers Raman spectroscopy （LTRS） has be, en applied to biology field. In the present article, the authors measured the spectra of liver cancer cells, para-cancer cells and normal hepatocytes using single cell laser tweezer Raman spectroscopy （LTRS） system and compared their average spectra changes. The results showed that the laser tweezers Raman spectroscopy could differentiate specimens of different pathological changes from liver tissue studied. The 1 070 and 1 266 cm^-1 peaks obtained from normal hepatocytes were more visible than the same two peaks obtained from liver cancer and para-cancer specimen. The 1 445 cm^-1 peak of normal hepatocytes was higher than that of liver cancer cells and para-cancer cells. It is known that the 1 070 cm^-1 peak represents hpids and nucleic acids, while 1 266 and 1 445 cm^-1 peaks represent lipids and proteins. So, these peak changes may directly reflect the changed biomaterials related to liver carcinogenesis. Thus, single cell laser tweezer Raman spectroscopy may be a nondestructive, rapid and good method to measure and analyze different pathological specimens from liver cancer.

关 键 词：人肝癌细胞 人肝正常细胞 拉曼光谱 

分 类 号：O657.3[理学—分析化学]