胸腺嘧啶在改进银溶胶溶液中的表面增强拉曼散射光谱信号与浓度的定量关系  

The Study on Linear Relationship between Concentration and Surface-Enhanced Raman Spectroscopy(SERS) Signal of Thymine in Improved Ag Sol

作　　者：张磊[1] 张玉兰[1] 张伟[1] 王文明[1] 杜一平[1] Yukihiro Ozaki 

机构地区：[1]华东理工大学分析测试中心,上海200237 [2]School of Science and Technology, Kwansei-Gakuin University, Sanda 669-1337, Japan

出　　处：《光谱学与光谱分析》2009年第7期1889-1891,共3页Spectroscopy and Spectral Analysis

基　　金：国家重点基础研究发展计划“973”项目(2007CB914104);上海市科委纳米专项项目(0652mm020)资助

摘　　要：尝试使用高分子材料作为银溶胶的稳定剂，以吡啶作为内标化合物研究胸腺嘧啶浓度与其SERS信号强度的关系。在胸腺嘧啶的浓度为2×10^-4～1×10^-3 mol·L^-1的范围内，谱峰强度之比与胸腺嘧啶的浓度之间呈现良好的线性关系，线性回归方程为y=3.143x＋0.348 7, 线性相关系数R^2=0.975 4。The authors used polymer as a stable medium to protect Ag ;sol to get a stable signal for the unstable Ag sol. Polyacrylic acid sodium was selected as the stable medium, which had a little effect on the SERS of thymine and made the SERS signal stable for thirty days. After aborative consideration and some trials, pyridine was picked as an internal standard for the quantitative detection of thymine. A linear correlation between the concentration of thymine and the ratio of thymine signal to pyridine signal intensity was obtained over a range of 2×10^-4～1×10^-3 mol·L^-1 with a correlation coefficient R2 of 0. 975 4. The regression equation is： y=3. 143x＋0. 348 7. The proposed method has the potential for quantitative analysis of the trace compounds having SERS effects.

关 键 词：表面增强拉曼散射 胸腺嘧啶 稳定剂 

分 类 号：O657.3[理学—分析化学]