利用拉曼光谱研究^(60)Co γ射线对EC9706细胞的辐射损伤  

Raman Spectra Study on Radiation Damage in EC9706 Cells by ^(60)Co γ-Rays

作　　者：齐健[1] 郭郑元[1] 张广水[1] 吴电勇[1] 唐伟跃[1] 

机构地区：[1]郑州大学物理工程学院,河南郑州450052

出　　处：《光谱学与光谱分析》2009年第7期1896-1900,共5页Spectroscopy and Spectral Analysis

基　　金：河南省科技攻关项目(0624410052)资助

摘　　要：将EC9706细胞经不同剂量60Co γ射线辐照后继续培养24 h。利用激光拉曼光谱分析EC9706细胞内部蛋白质、核酸、脂类等生物大分子的构象和含量变化。分析结果发现,各剂量组拉曼谱的峰强和频移与空白对照组之间存在较大的差异,主要表现是蛋白质酰胺Ⅲ带1 244 cm-1谱带在中等剂量组(4、5Gy)β折叠结构向无规卷曲转化;色氨酸残基的吲哚环振动谱带1 341 cm-1在各剂量组中出现不同程度的红移;782cm-1谱带在大剂量组(7、8Gy)红移2~3 cm-1,说明大剂量γ射线辐照导致DNA中的磷酸二酯(O—P—O)基团的非氢键化程度增强。脂类的CH2和CH3弯曲振动谱带1 446 cm-1在2、4Gy组蓝移4 cm-1,其他剂量组频移不大,这与60Co γ射线对EC9706细胞的生物膜造成一定损伤有关。拉曼特征峰在不同剂量组中的变化,为进一步研究60Coγ射线辐照损伤EC9706细胞的最佳剂量提供了一定实验依据。Raman spectrum was used to study the structure and content of protein, nucleic acid and fat, while EC9706 cells irradiated by ^60Coγ-ray were cultivated for 24 h. The results showed that for spectrum intensity and frequency deviation, there were big differences between each exposure group and control group. For the 1 244 cm^-1 peak of amide Ⅲ, β folder changed to disordered conformations in the middle dose （4, 5Gy） groups. The 1 341 cm^-1 peak of v （the indole ring of Trp） was red-shifted in every dose group. There was a 2-3 cm^-1 red shift at the 782 cm^-1 peak in the big dose groups （7, 8Gy）. It was showed that the non-hydrogenation of vs（PO2^-） was strengthened due to big dose γ-rays radiation. There was a 4 cm^-1 blue shift at the 1 446 cm^-1 peak of 8 （CH2, CH3 ）. It maybe resulted from ^60Coγ-rays＇ damage to the film of EC9706 cells. The preferable dose of ^60Coγ-rays may be found by analyzing the variety of the above-mentioned peaks in some dose groups.

关 键 词：60Co Γ射线 人食管癌细胞 拉曼光谱 剂量 

分 类 号：O657.3[理学—分析化学]