贵州省鸡群中禽白血病病毒J亚群感染的调查  

Investigation on Infection of Subgroup J Avian Leukosis Virus in Different Flocks of Guizhou Province

作　　者：罗明星[1] 周碧君[2] 李永明[2] 汪德生[2] 史开志[1] 杨峰 赵航[1] 袁圣[1] 付鸣剑[1] 

机构地区：[1]贵州大学动物科学院,贵州贵阳550025 [2]贵州大学动物疫病研究所,贵州贵阳550025 [3]贵阳市农业局兽防站,贵州贵阳550000

出　　处：《中国家禽》2009年第11期15-18,23,共5页China Poultry

基　　金：贵阳市农业局科学技术计划项目项目合同号[2008动防-01];贵阳市农业局科学技术计划资助项目([2006]5001号);贵州省农业厅兽医科技计划项目(2008-06)

摘　　要：采用禽白血病J亚群抗体检测试剂盒对4个不同肉种鸡场的血清样本进行检测,发现ALV-J抗体阳性率平均达到16.52%(3.33%～30.0%)。对出现肿瘤病变的显性型病鸡进行病理组织学检查,在肝、肾、脾组织中观察到大量增生的、含嗜酸性颗粒的髓细胞样瘤细胞。针对J亚群gp85基因部分序列进行PCR扩增,从ALV-J抗体阳性鸡的肝病料扩增出一条545bp DNA带,而ALV-J抗体阴性鸡的样本显现阴性反应。将扩增的gp85基因序列与国内外J亚群毒株相应序列进行比较,结果核苷酸序列的同源性分别达到94.8%～96.5%和95.5%～96.8%。综合血清学、病理组织学变化和病原分子生物学鉴定结果,证实贵州省肉种鸡群中存在ALV-J亚群病毒感染。The serum samples were collected from 4 different breeding broiler farms and detected with avian leukosis virus antiboby test kit-subgroup J （ALV-J）. The positive rate of ALV-J antibody averaged 16.52% in different broiler flocks （3.33%-30,0%）.The histopathological examination for the sick or dead chickens which manifested tumor lesions in visceral organs showed a lot of myelocyte-like tumor cells filled with acidphilus granules in liver,kidney and spleen tissues, gp85 gene of subgroup-J virus was amplified by PCR and a 545 bp fragment was amplified from livers of chickens which were positive to ALV-J antibody. No fragment was observed from samples of ALV-J antibody negative chickens. Compared with the relative sequence of subgroup J isolates published in GenBank,the homology of nucleotide sequences reached 94.8%-96.5% and 95.5%-96.8% respectively. These results demonstrated ALV-J infection in breeding broiler flocks of Guizhou province.

关 键 词：禽白血病 J亚群 血清学 组织病理 PCR 肉鸡 

分 类 号：S852.65[农业科学—基础兽医学] S831.4[农业科学—兽医学]