高亲和力抗膀胱癌单链抗体筛选及亲和力测定  

Gene Sequence Panning of High and Special Affinity of Humanized Single-Chain Antibody Against Human Bladder Carcinoma

作　　者：晁开[1] 黄华樑[2] 郭霭光[3] 

机构地区：[1]甘肃省科学院,甘肃兰州730000 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《甘肃科学学报》2009年第2期69-72,共4页Journal of Gansu Sciences

基　　金：国家863计划资助项目(863-102-09-04-01)

摘　　要：以膀胱癌细胞系BIU-87膜抗原为亲和底物,从抗膀胱癌噬菌体人源化单链抗体库中筛选出4个高亲和力改形单链抗体.ELISA结果和NH4SCN洗脱法数据显示,它们对膀胱癌细胞膜抗原的亲和活性显著高于鼠源单链抗体.同时以多种蛋白检验了其亲和作用的特异性.Through 3 rounds of panning with BIU-87 cell associated membrane antigen,we screened the phagemid library for high affinity humanized scFv and 4 clones were obtained as a result. Data from measurement of thiocyanate elution ELISA showed that they had more binding-activity to BIU-87 cell associated membrane antigen than that of starting mouse scFv. And then, we tested and verified their special affinity by sorts of other proteins .

关 键 词：抗膀胱癌单链抗体 特异性高亲和力 筛选 

分 类 号：R392.11[医药卫生—免疫学]