切尔诺贝利事故辐射受照人员淋巴细胞核的形态变化  

Morphological Change of Lymphocytes Nucleus in Irradiated Person from Chemobyl Nuclear Accident.

作　　者：李雨[1] Kravtsov V.Yu Aleksanin S.S 

机构地区：[1]上海第二军医大学海医系放射医学教研室,上海200433 [2]俄罗斯急救与放射医学中心

出　　处：《中国辐射卫生》2009年第2期129-131,共3页Chinese Journal of Radiological Health

基　　金：国家自然科学基金面上项目(编号:30270421)

摘　　要：目的观察受照射的切尔诺贝利事故清理工作者外周血淋巴细胞核特殊形态变化。方法选取切尔诺贝利事故清理工作者作为试验组,采用常规方法取指尖外周血制备涂片,空气干燥,对氨基苯甲酸混合,Giemsa法染色,光镜下计数每人500个细胞样本,观察记录不同特殊形态核发生率,进行统计学处理。结果大多数受照射对象都出现较多带有尾状突起的淋巴细胞。根据这些核上突起的尾状物形态特征将其分为16种类型并分别记录了每种尾状突起细胞的发生率并进行分布率分析。结论受照射淋巴细胞核尾状突起变化可能是一种有意义的受照射标记物,对于更加详尽获取辐射生物效应信息,回顾性研究辐射剂量效应可能具有理论和实践价值。Objective To study morphological change of lymphocyte nuclei in irradiated persons in Chernobyl nuclear accident. Methods Select peripheral blood lymphocytes from irradiated person in the Chernobyl nuclear accident as experiment group to be stained by routine Giemsa method and take 500 cells from each under microscope as samples, observe and record the different pattern morphological changes of lymphocytes nuclei. Results There are lymphocytes nuclei which have tail in the irradiated persons. According to the different morphological features, describe and record 16 different patterns for the lymphocytes nuclei. Conclusion The lymphocytes nuclei which have tail in the irradiated persons and probably a marker of irradiation.

关 键 词：切尔诺贝利核事故 辐射 淋巴细胞 

分 类 号：Q691[生物学—生物物理学]