连翘酯苷对体外培养细胞的毒性研究  

Study on the Toxicity of Forsythiaside on Cell Culture in Vitro

作　　者：武聚富[1,2] 张中文[1] 况玲[2] 沈红[1] 陆彦[1] 吴国娟[1] 

机构地区：[1]北京农学院,北京102206 [2]新疆农业大学,乌鲁木齐830052

出　　处：《中国兽药杂志》2009年第6期4-8,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金(30771625);北京市自然科学基金(6092004);北京市属高校人才强教计划资助项目(PHR2009-07135)

摘　　要：探讨了连翘酯苷对体外培养细胞的毒性,以期为人们更好地研究利用连翘酯苷提供实验依据。实验采用细胞病变法观察连翘酯苷对猪肾细胞(PK-15)、猴肾细胞(Marc-145)、鸡胚肾细胞(CEK)、鸡外周血淋巴细胞(PBL)等4种细胞的形态损伤;用MTT法检测连翘酯苷作用这4种细胞后的细胞活性和细胞数量的变化。根据这两种方法的结果计算出连翘酯苷对这4种细胞的半数致死量。实验结果显示高剂量的连翘酯苷可致细胞发生病变,而低剂量组的细胞形态优于对照组。经SPSS16.0统计,连翘酯苷对PK-15、Marc-145、CEK3种细胞的半数致死量分别为137.8、87.1、384.4μg/mL,对PBL应用到中华人民共和国兽药典(2000年版)规定药量的10倍剂量,结果未发现致死性。因此,适当合理的连翘酯苷用量对体外培养的细胞没有毒性,反而有一定的维持细胞形态的营养作用和刺激细胞增殖的作用。Forsythia is one of the most important heat - clearing and detoxicating drugs in Chinese medicine. Aiming to investigate its toxicity on cell culture, four kinds of cells were selected, namely, pig kidney cells （PK -15 ）, monkey -kidney cells （Marc -145 ）, chicken embryo kidney cells （CEK） and chicken lymphocytes （PBL）. Through this research, this article wished to offer experimental basis for exploring and making better use of forsythiaside. This experiment observed the eytopathic effect （CPE） of all the cells mentioned above except PBL by inverted microscope and examined the eytoactive and the change of cell quantities by MTT. Based on the results of these two methods, the article calculated the median lethal dose （ LD50 ） of forsythiaside. The results showed that high doses of forsythiaside might render CPE, while the low monitoring team＇ s cell - morph was superior to the control group. According to the statistical analysis by SPSS 16.0, the LD50 of the forsythiaside on PK - 15, Marc - 145 and CEK was 137.8 μg/mL, 87. 1 μg/mL, 384.4 μg/mL. 95% confidence interval was 128.7 - 151.7 μg/mL for PK - 15, 73.7 - 100.4 μg/mL for Marc - 145 and 334.8 -469.2 μg/mL for CEK. There was no damage on PBL by using 10 times forsythiaside than Veterinary Pharmacopoeia of the People＇ s Republic of China. This research came to the conclusion that appropriate and rational drug sustain cellular morphous and stimulate cell reduplication rather than cause toxicity on the four had been selected in the experiment.

关 键 词：连翘酯苷 细胞培养 毒性 

分 类 号：S853.74[农业科学—临床兽医学]