广东土牛膝HPLC指纹图谱的研究  

Fingerprint of Eupatorium chinense by HPLC

作　　者：张军[1] 张晓琦[2] 汤毅 林雪颖[1] 邓鲲鹏[1] 

机构地区：[1]广州中医药大学新药开发研究中心,广东广州510405 [2]暨南大学中药及天然药物研究所,广东广州510632 [3]广州中大创新公司,广东广州510070

出　　处：《中草药》2009年第6期964-967,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的采用HPLC法对广东土牛膝进行指纹图谱研究,为广东土牛膝药材质量控制提供依据。方法实验采用Kromasil C18柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相梯度洗脱,体积流量1.0 mL/min,柱温25℃,检测波长230 nm。结果建立广东土牛膝药材HPLC指纹图谱,确定13个共有峰,确定其相对保留时间和相对峰面积。结论在选定的条件下所建立的指纹图谱为广东土牛膝药材质量控制提供依据。Objective To establish the HPI.C fingerprint of Eupatoriurn chinense. Methods The analysis was performed on a Kromasil C18 column （250 min×4.6 mm, 5 μm） with acetonitrile-water as mobile phase in a gradient mode. The flow rate was 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was 230 nm. Results The fingerprint of E. chinense with common 13 peaks was established. The relative retention time and the ranges of relative area of the common peaks were determined. Conclusion The established fingerprint could be used for the quality control of E. chinense.

关 键 词：广东土牛膝 HPLC指纹图谱 泽兰素 

分 类 号：R282.7[医药卫生—中药学]