枣针刺长度的数量性状位点定位与分析  

Analysis of QTL for Needle Length in Chinese Jujube

作　　者：齐靖[1] 董祯[2] 申连英[1] 毛永民[1] 李艳辉[1] 刘杰[1] 王晓玲[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北女子职业技术学院,石家庄050091

出　　处：《园艺学报》2009年第6期807-813,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30370994);国家‘十一五’科技支撑计划项目(2006BAD01A1701);河北省自然科学基金项目(C2007000521)

摘　　要：以150株‘冬枣’ב临猗梨枣’F1代群体为试材,调查了4种类型枣针刺(中心干上长针刺、中心干上短针刺、二次枝上长针刺和二次枝上短针刺)长度的分离情况。结果表明,4种性状表现为连续分布,具有数量性状的典型特征,且4个性状两两之间存在极显著正相关关系,在F1代群体中趋向于同步分离。使用分子连锁图谱进行数量性状位点(QTL)分析,共检测到控制枣针刺长度的QTL26个,包含控制中心干上长针刺长度10个,控制中心干上短针刺长度5个,控制二次枝上长针刺长度8个和控制二次枝上短针刺长度3个。各QTL的LOD值在2.53～5.27之间,可解释8.2%～44.2%的表型变异。26个QTL分布在分子连锁图谱的7个连锁群中,其中4个连锁群上的5个位点同时定位了2～3个类型的QTL,证实部分基因在对不同类型针刺长度的控制上存在一因多效。The length of 4 types needle in Chinese jujube （ Ziziphus jujuba Mill. ）, include the long needle on trunk （TLN）, the short needle on trunk （TSN）, the long needle on branch （BLN） and the short needle on branch （BSN） were evaluated by using an F1 population consisting of 150 individuals, which was derived from a cross between ＇ Dongzao＇ and ＇ Linyilizao＇. In the F1 population, the 4 traits showed continuous variation, and there were significant correlation between traits that they were tend to synchronize separation with each other. By using interval mapping method, a total of 26 QTLs were detected, include 10 for TLN, 5 for TSN, 8 for BLN and 3 for BSN, phenotypic variation explained by each QTL ranged from 8.2% to 44. 2%, and its LOD value within 2. 53 - 5.27. Among those QTLs, 12 QTLs which controlling different type needle length were mapped on the same intervals, forming 5 elusters in 4 linkage groups that could control more than one trait, suggesting that there be some pleiotropic effects genes could control the length of different types needle.

关 键 词：枣 针刺长度 F1群体 相关性 数量性状位点(QTL) 

分 类 号：S665.1[农业科学—果树学] Q75[农业科学—园艺学]