脱锻炼期间白皮松针叶的电阻抗图谱参数和生理指标的相关性  

The Relationship Between Parameters of Electrical Impedance Spectroscopy and Some Physiological Parameters in Pinus bungeana Zucc. Needles During Dehardening

作　　者：董胜豪[1] 张钢[1] 卻书鹏 祝良[2] 靳秀梅 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]北京十三陵林场苗圃,北京102200

出　　处：《园艺学报》2009年第6期891-897,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30640035);河北农业大学留学回国人员科研启动基金项目(2004-2006);河北农业大学新兴学科发展基金项目(2006-08)

摘　　要：2007年3—5月,对北京十三陵苗圃的北京蟒山、山西孝义和甘肃两当3个种源的9年生白皮松(Pinus bungeana Zucc.)针叶进行了电阻抗图谱(EIS)分析,测定其未冷冻针叶的EIS参数,以及超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、可溶性蛋白质、脯氨酸和干物质含量等生理指标的变化,比较脱锻炼期间不同种源白皮松针叶的EIS参数和生理指标之间的相关性。结果表明:(1)脱锻炼期间未冷冻针叶的EIS参数β和胞外电阻率re与针叶的脯氨酸含量之间存在负相关;细胞膜时间恒量τm与针叶的可溶性蛋白质含量存在正相关;(2)胞内电阻率ri与MDA,τm与干物质含量的变化趋势相同,而re和β在脱锻炼前期与可溶性蛋白质含量的变化趋势类似;(3)冷冻处理后用τm和re求出的抗寒性与脯氨酸含量相关。From March to May 2007, the electrical impedance spectroscopy （EIS） was analyzed on oneyear-old needles of Pinus bungeana Zucc. in a 9-year provenance field trial at the Nursery in Beijing Ming Tombs Forestry Center with provenances of Mangshan in Beijing, Xiaoyi in Shanxi and Liangdang in Gansu. The activities of superoxide dismutase （ SOD ） and peroxidase （ POD ）, the contents of malondialdehyde （MDA） , soluble proteins and proline, and dry weight percentage in needles were tested with different provenances. The relations between parameters of the EIS and tested physiological parameters were analyzed. The results indicated that （ 1 ） during dehardening, EIS parameters β and specific extracellular resistance τe in nonfrost-exposed needles correlated negatively with the content of proline; and there was a positive relation between the membrane time constant τm and the content of soluble protein; （2） Similar changing patterns were found between the specific intracellular resistance τi and the content of MDA ; Between the τm and the percentage of dry weight, and between τe, β and soluble proteins in the early stage of dehardening; （3） The frost hardiness assessed by ro and Tm after artificial freezing treatment correlated to the content of proline.

关 键 词：白皮松 脱锻炼 生理指标 电阻抗图谱 

分 类 号：S687.1[农业科学—观赏园艺] Q945.78[农业科学—园艺学]