秋水仙素诱导乌拉尔甘草同源四倍体的研究  

Induction of Autotetroploid of Glycyrrhiza uralensis Fisch with Colchicine

作　　者：葛淑俊[1] 袁静娅[1] 武晓阳[1] 郑丽 

机构地区：[1]河北农业大学／河北省作物种质资源重点实验室,河北保定071001 [2]河北巨微生物工程有限公司,河北保定071001

出　　处：《作物杂志》2009年第3期8-11,共4页Crops

基　　金：河北省科技支撑计划(09276424D);河北省自然科学基金(08B024);河北农业大学博士科研发展基金

摘　　要：在组织培养条件下,用秋水仙素溶液浸泡乌拉尔甘草试管苗(2n=2X=16)茎尖进行同源四倍体诱导,比较了不同秋水仙素浓度和不同处理时间对存活率、变异率的影响,并对加倍植株的鉴定和纯合四倍体的分离方法进行了研究。结果表明,秋水仙素浓度为0.1%,浸泡时间为12h时,茎尖的存活率和变异率分别为52.3%和22.1%,诱导率较高,并通过保卫细胞和根尖染色体数目鉴定,成功获得了甘草同源四倍体(2n=4X=32),为开展甘草多倍体育种奠定了物质和技术基础。In the paper stems in vitro of Glycyrrhiza uralensis Fisch （2n =2X = 16） were used to induce autotetroploid with colchincine. The effect of colchicine concentration and treatment time was discussed and the purification of mosaic of Glycyrrhiza uralensis and the identification of doubled individuals were studied. The results showed that the variation and doubling percentage could reach to 52.3% and 22%, respectively, by soaking stems with 0.1% colchicines for 12h. The autotetroploid plants were obtained through identification of the chromosome and guard cell of stoma （2n = 4X = 32）.

关 键 词：乌拉尔甘草 同源四倍体 秋水仙素 嵌合体 

分 类 号：S567.71[农业科学—中草药栽培]