桃褐腐病菌拮抗细菌的分离筛选与鉴定  

Isolation and Screening of Antagonistic Bacteria against Monilinia fructicola

作　　者：胡海文[1] 杨海清[1] 王朋[1] 刘素花[1] 赵晓燕[1] 刘正坪[1] 

机构地区：[1]北京农学院植物科学技术系,北京102206

出　　处：《中国农学通报》2009年第12期195-200,共6页Chinese Agricultural Science Bulletin

基　　金：北京市教委资助项目"拮抗细菌对桃褐腐病的生防机制研究"(KM200710020003);北京市教委资助项目"设施新生有害生物防控技术研究平台"(PXM2008-014207-055664);北京市农委资助项目"动植物重要病害绿色防治新技术的试验示范"(20060119);北京农学院引进人才科研启动基金"桃褐腐病菌细胞壁降解酶基因克隆及在致病过程中的作用";北京市科委区县专项2006"植物源杀螨活性物质研究"

摘　　要：为了筛选和鉴定桃园土壤中对桃褐腐病菌有拮抗作用的细菌,采用对峙培养法,16SrDNA基因分析以及生理生化反应等方法。从北京平谷区桃园土样中分离的42株细菌中筛选出了11株对桃褐腐病菌(Monilinia fructicola)有明显抑制作用的拮抗细菌。其中F3和Y4的拮抗效果最好,抑菌带的宽度分别为13.83mm和14.67mm,F1和F4抑菌效果次之,抑菌带宽度分别为11.83mm和12.00mm。在显微镜下观察发现,F3和Y4对桃褐腐病菌的菌丝有致畸作用,并且F3和Y4的培养滤液还能够抑制桃褐腐病菌分生孢子的产生和萌发。经鉴定表明:F1、F3、F4和Y4均为枯草芽孢杆菌(Bacillus subtilis)。Confrontation incubation, 16s rDNA analyse and physiological and biochemical reactions were adopted to screen and identificate the antagonistic bacteria against Monilinia fructicola from peach orchard soil. 1 lout of 42 bacteria strains isolated from peach orchard soil samples of Pinggu strict, Beijing showed obvious antagonism against Monilinia fructicola. F3 and Y4 had the best antagonistic effects with the antagonistis strip width of 13.83mm and 14.67mm separately. The antagonistic effects of F1 and F4 came second with the anatagonistis strip width of 11.83 mm and 12.00 mm. F3 and Y4 had teratogenicity to the mycelium morphology of Moniliniafructicola. And the culture filtrate of F3 and Y4 could inhibit the conidial formation and germination of M. fructicola. F1 , F3, F4 and Y4 were identified as Bacillus subtilis.

关 键 词：拮抗细菌 桃褐腐病菌 分离筛选 16SrDNA 生理生化反应 

分 类 号：S436.621.13[农业科学—农业昆虫与害虫防治]