重组血管内皮生长因子_(165)腺相关病毒对心肌梗死大鼠心功能的影响  

Effect of intramyocardial injection of rAAV-hVEGF_(165) on cardiac function of rats with myocardial infarction

作　　者：李曦铭[1] 王超[2] 王林[3] 韩瑞发[4] 刘利维[4] 丛洪良[1] 

机构地区：[1]天津市胸科医院,天津300051 [2]天津市南开医院 [3]天津医科大学第二医院 [4]天津市泌尿外科研究所

出　　处：《中华老年心脑血管病杂志》2009年第6期444-447,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：天津自然科学基金(05YFJMJC12500)

摘　　要：目的探讨直接心肌注射基因重组内皮生长因子_(165)腺相关病毒(rAAV-hVEGF_(165))对急性心肌梗死大鼠心功能的影响。方法选择雄性SD大鼠81只,将0.1 ml生理盐水或rAAV-hVEGF_(165)分3点注射于梗死交界处心肌内。根据注射药物的不同随机分为4组:假手术组(15只),心肌梗死组(25只),生理盐水组(25只),血管内皮生长因子(VEGF)组(16只)。注射4周后测定心肌组织VEGF含量、超声心动图参数、梗死面积、微血管数量、心钠素水平。结果 VEGF组大鼠心肌梗死面积较心肌梗死组和生理盐水组明显减小(P<0.05),左心室射血分数明显高于心肌梗死组和生理盐水组(P<0.01),心肌组织VEGF含量较心肌梗死组和生理盐水组有所增加。血管计数显示,VEGF组大鼠较其他3组有更多的新生血管形成(P<0.01)。结论直接心肌注射rAAV-hVEGF_(165)能够明显改善急性心肌梗死大鼠的心功能,缩小梗死面积,促进心肌内新血管的形成。Objective To determine the effects of intramyocardial injection of rAAV-hVEGF165 on cardiac function in a rat model of acute myocardial infarction（AMI）. Methods Eighty-one male SD rats were included. 0. 1 ml of rAAV-hVEGF165 or saline was intramyocardially injected into three different sites at the border zone of infarction. The models were divided randomly into four groups：group sham operation （n = 15） ; group NS （n = 25） ; group MI （n = 25） ; group VEGF （n = 16）. Echocardiogram data, infarct size, intramyocardial microvessels, VEGF in myocardium were measured 4 weeks after injection. Results Infarct size in group VEGF was significantly smaller than that in group MI and group NS （P 〈0.01）. Echocardiogram data revealed that improvement of ejection fraction（LVEF%） was greater in group VEGF than in group MI and group NS （P 〈0. 01）. Vessel count showed that there were more intramyocardial vessels in group VEGF than in other 3 groups （P〈 0.01）. Conclusions The research shows that intramyocardial injection of rAAV-hVEGF16s is capable of significantly improving cardiac function, decreasing infarct size,promoting myocardial neovascularization in a rat model of acute myocardial infarction.

关 键 词：心肌梗塞 血管内皮生长因子A 心钠素 基因疗法 

分 类 号：R542.22[医药卫生—心血管疾病]