酸性铬蓝K退色光度法测定更昔洛韦的含量  

Determination of ganciclovir by acid chrome blue K fading spectrophotometry

作　　者：江虹[1] 张华[1] 谢发萍[1] 

机构地区：[1]长江师范学院化学及环境科学系,重庆408100

出　　处：《化学试剂》2009年第6期441-443,共3页Chemical Reagents

基　　金：重庆市教委科技基金项目(KJ051304)

摘　　要：在pH5．6～6．8的酸性条件下，更昔洛韦与酸性铬蓝K发生退色反应，生成玫瑰红色离子缔合物，最大退色波长位于522nm，线性范围为0．1～7．2mg／L，表观摩尔吸光系数（￡）为4．89×10^-4L·mol^-1·cm^-1，更昔洛韦在一定浓度范围内遵从朗伯一比尔定律，由此建立了测定更昔洛韦的光度分析法。该法用于实际样品的测定，回收率在99．6％～100．5％。Under the acidic condition at a pH value of 5.6 - 6.8, acidic chrome blue K reacted with ganciclovir to form a rose red ion-association complex. The maximum fading wavelength was at 522 nm. The Beer＇s law was obeyed in the concentration range of 0.1 - 7.2 mg/L, and the apparent molar absorption coefficient （ E ） was 4.89×10^-4L·mol^-1·cm^-1. Thereby a new spectrephotometric method was developed for the determination of ganciclovir. The method has been applied to the determination of genciclovir samples with the recovery rate equating to 99.6% - 100.5%.

关 键 词：更昔洛韦 酸性铬蓝K 分光光度法 

分 类 号：O657.32[理学—分析化学]