氨茶碱对支气管哮喘气道重塑大鼠肺组织MMP-9和TIMP-1含量的影响  

The effect of aminophulline on the content of MMP-9,TIMP-1in lung tissue of rats with bronchia asthma undergoing airway remolding

作　　者：段旭东[1] 赵辉[1] 于文涛[2] 王晓媛[1] 张雅兰[1] 

机构地区：[1]河北医科大学第二医院,石家庄市050000 [2]河北医科大学中医学院

出　　处：《河北医药》2009年第9期1027-1028,共2页Hebei Medical Journal

摘　　要：目的观察氨茶碱对支气管哮喘气道重塑大鼠气道形态学和基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制物-1(TIMP-1)的影响。方法24只SD大鼠随机分为正常组、模型组、治疗组,每组8只,除正常组外以卵蛋白致敏并吸入激发法制备大鼠哮喘模型,治疗组、模型组从第1次哮喘激发开始(造模第3周)分别给予氨茶碱、0.9%氯化钠溶液灌胃给药,1次/d,用药4周处死大鼠,取肺组织HE染色,用彩色图像分析仪测量支气管壁面积、支气管平滑肌面积,用ELISA双抗体夹心法测定肺组织MMP-9和TIMP-1含量。结果与正常组比较,模型组大鼠支气管壁面积、平滑肌面积、肺组织MMP-9和TIMP-1含量及MMP-9/TsMP-1比值增加明显(P<0.01)。治疗组大鼠支气管壁面积、平滑肌面积、肺组织MMP-9和TIMP-1含量及两者比值显著降低(P<0.05)。结论氨茶碱可通过降低肺组织MMP-9和TIMP-1含量、调节两者比值,以抑制哮喘大鼠气道壁、平滑肌增厚,减缓支气管哮喘大鼠气道重塑。Objective To observe the effect of aminophulline on the content of MMP-9, TIMP-1 in lung tissue of rats with bronchia asthma undergoing airway remolding. Methods 24 SD rats were randomly divided into normal group, model group, aminophylline treatment group （35 mg/kg）, 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and long-term inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation. After 4-week treatment, the rats were killed to obtain lung tissue for staining with HE. A computer assisted image analysis system was used to determine the area of bronchus wall and smooth muscle. The content of MMP-9 and TIMP-1 in lung tissue were detected by ELISA double antibody sandwich method. Results The area of bronchus wall and smooth muscle, the content of MMP-9 and TIMP-1 and the value of MMP-9/TIMP-1 in lung tissue were increased in model group than in normal group （ P 〈0.01 ）, the area of bronchus wall and smooth muscle, the content of MMP-9 and TIMP-1 and the value of MMP-9/TIMP-1 in lung tissue were decreased in treatment group （ P 〈 0. 01 ）. Conclusion Aminophylline can decrease the product of MMP-9 and TIMP-1 in lung tissue and regulate the relative value of both to inhibit air passage thickening and smooth muscle hyperplasia to restrain airway remolding.

关 键 词：氨茶碱 支气管哮喘 气道重塑 基质金属蛋白酶-9基质 基质金属蛋白酶抑制物-1 

分 类 号：R562.25[医药卫生—呼吸系统]