探针5-硝基水杨酸同步荧光法测定生物样品中蛋白质  

Determination of Proteins in Biology Samples by Synchronous Fluorescence Technique with 5-Nitrosalicylic Acid as a Probe

作　　者：崔凤灵[1] 秦利霞[1] 李芳[1] 郝二军[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《应用化学》2009年第6期730-733,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20673034);高等学校博士学科点专项科研基金(20060476001);河南省青年骨干教师资助计划(200470);新乡市科技发展计划(07S038)资助

摘　　要：在模拟体液离子强度下,基于5-硝基水杨酸（5-NSA）与人血清白蛋白（HSA）相互作用生成复合物,导致血清白蛋白的内源荧光产生特异性变化,而建立了以5-NSA为分子探针,用固定波长同步荧光光谱分析测定蛋白质的新方法。体系同步荧光光谱特征及强度受Δλ、反应介质、反应温度等因素的影响。结果表明,在最佳实验条件下,体系的同步荧光强度（ISF）与人血清白蛋白在3.2×10^-8-6.4×10^-8mol/L的质量浓度范围内呈良好的线性关系,检测限为1.7×10^-8mol/L（n=11）。对血清、尿样和唾液样品进行了测定,回收率在99.96%-100.04%之间。5-Nitrosalicylic acid can react with human serum albumin （HSA） to form a complex. Based on this, a new method for the determination of proteins by synchronous fluorescence spectra under simulated physiological conditions was established with 5-nitrosalicylic acid as a fluorescence probe. The spectral characterization and the intensity of synchronous fluorescence were related to the value of AA, reaction medium and reaction temperature is. Experimental results show that under the optimum conditions, the synchronous fluorescence intensity of the system is in proportion to the concentration of HSA in a range of 3.2 × 10 ^-8 - 6. 4 × 10^-6 mg/L. The detection limit is 1.17 × 10^-8 mol/L（n = 11 ）. The determination of proteins in human serum, urine and saliva samples is performed with satisfactory results, the recovery is within a range of 99. 96% - 100. 04%.

关 键 词：硝基水杨酸 人血清白蛋白 同步荧光光谱 测定 

分 类 号：O657.3[理学—分析化学]