粪便联合检测致泻病原体在婴幼儿腹泻中的应用  

Application of Combined Detection of Fecal Pathogens in Infantile Diarrhea

作　　者：陈如昌[1] 王利健[1] 

机构地区：[1]义乌市中心医院检验科,浙江义乌322000

出　　处：《中华医院感染学杂志》2009年第12期1610-1612,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨粪便联合检测致泻病原体在婴幼儿腹泻中的应用价值。方法539份粪便标本同时做细菌培养和3种致泻病原体抗原的检测,肠致病性大肠埃希菌(EPEC)采用分离培养法,对可疑菌株作血清凝集试验,确定血清型;轮状病毒、肠道腺病毒、空肠弯曲杆菌采用乳胶凝集试验测定其抗原。结果腹泻患儿中轮状病毒检出率达33.0%,空肠弯曲杆菌的检出率7.2%,EPEC的检出率为1.5%,肠道腺病毒的检出率0.7%;检测阳性者的男女比例1.6∶1。结论婴幼儿感染性腹泻的病因主要是轮状病毒和空肠弯曲杆菌,EPEC和肠道腺病毒感染率较低。联合检测致泻病原体对诊断婴幼儿腹泻有应用价值。OBJECTIVE To investigate the application value of combined detection of fecal pathogens in infantile diarrhea. METHODS The Escherichia coli was detected by potato-dextrose agar（PDA） medium method and serum agglutination test was used to determine the serotype. The rotavirus, enteric adenovirus and Campylobacterjeju- ni were detected with latex agglutination test antigens. RESULTS Among the diarrhea children, the detection rate of rotavirus, C. jejuni to their, E. coli 1. 5%, and adenovirus was 33. 0%, 7. 2%, 1. 5% and 0. 7%, respectively. The proportion of male and female detected positive was 1.6 ： 1. CONCLUSIONS The main etiologies of infantile infectious diarrhea are rotavirus and C. jejuni. The infection rate of E. coli and enteric adenovirus is lower. Therefore, combined detection of pathogens causing diarrhea is valuable to diagnose the infantile diarrhea.

关 键 词：联合检测 腹泻 病原体 轮状病毒 肠致病性大肠埃希菌 

分 类 号：R378[医药卫生—病原生物学]