α-硅酸钙和β-硅酸钙的体外体内降解性研究  

Comparative Study the Degradation of α-CaSiO_3 and β-CaSiO_3 Bioactive Bioceramics in Vivo and in Vitro

作　　者：刘勇[1] 王臻[1] 林开利[2] 常江[2] 刘鹏[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]中国科学院上海硅酸盐研究所,上海200050

出　　处：《科学技术与工程》2009年第10期2569-2573,2582,共6页Science Technology and Engineering

基　　金：国家自然科学基金重点项目(30730034);上海市科委项目(07ZR14127)资助

摘　　要：研究α-CS和β-CS多孔硅酸钙生物陶瓷的体内、体外降解性能,并与β-磷酸三钙(β-TCP)多孔生物陶瓷比较。多孔生物陶瓷由添加造孔剂和高温烧结工艺制备得到。体外实验:将陶瓷样品在三羟甲基胺基甲烷-盐酸(Tris-HCl)缓冲溶液中浸泡1、3和7天后,通过测量材料的失重率来评价材料的降解性;体内实验:在新西兰大耳白兔股骨髁骨缺损处植入人工骨陶瓷,于术后第1周、4周、8周和12周收集标本,进行组织学观察并计算残余材料的面积百分比,分析材料的体内降解性能。体外降解性实验结果显示,三种材料的降解率β-CS>α-CS>β-TCP。β-CS和α-CS间无统计学差异(P>0.05),各时间点两种材料均与β-TCP有统计学差异(P<0.05)。体内降解性实验结果显示,三种材料随时间延长都表现出降解,降解率β-CS>α-CS>β-TCP,但是统计学结果显示没有差异(P>0.05)。β-CS和α-CS多孔生物活性陶瓷的降解性均明显优于β-TCP多孔生物陶瓷。研究结果显示β-CS和α-CS多孔生物陶瓷均有望作为硬组织修复和骨组织工程支架材料使用。The degradability of the α-CS and β-CS bioactive macroporous bioceramic in vitro and in vivo were comparatively studied, and the results were compared with β-tricalcium phosphate （ β-Ca3 （ PO4 ）2, β-TCP） macroporous bioceramics. The macroporous α-CS, β-CS and β-TCP bioceramics were obtained after sintering at high temperature . The degradability of macroporous bioceramics in vitro was determined by their weight loss percentage in Tris - HCl buffer solution after soaking for 1, 3 and 7 days. In the experiment in vivo, the ceramic samples were implanted in a rabbit condyles of femur defect model, and the specimens were harvested after 1,4 , 8 and 12 weeks, respectively. Degradation of the grafts were evaluated by the means of histological and histomor- phometry after 1, 4 , 8 and 12 weeks of implantation. The experiment in vitro result showed that the degradation rate was β-CS 〉 α-CS 〉 β-TCP. There is no obviously difference in statistics between β-CS and α-CS （ P 〉 0.05 ）. There is obviously difference in statistics between β-TCP and CS. In the experiment in vivo, the histological and histomorphometry results showed that the degradation rate was β-CS 〉 α-CS 〉 β-TCP, but there is no obviously difference in statistics. The present studies showed that the degradability of the porous β-CS and α-CS ceramics is much faster than that of β-TCP. Therefore, both of the β-CS and α-CS macroporous bioceramics could be used as bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.

关 键 词：骨缺损 骨移植 骨再生 硅酸钙 磷酸三钙 人工骨 降解 

分 类 号：R318.08[医药卫生—生物医学工程]