乙型肝炎病毒感染者CD8^+T淋巴细胞T细胞抗原受体Vβ基因互补决定区3克隆化特征的研究  

Research on cloning characteristics of complementary determining region 3 distinct in T cell antigen receptor Vβ gene of CD8+~T cell of patients infected with hepatitis B virus

作　　者：宋玉国[1] 张吉林[2] 黄建文[1] 宋秀燕[1] 

机构地区：[1]北华大学附属医院临床免疫研究室,吉林吉林132011 [2]北华大学医学检验学院分子生物学教研室,吉林吉林132013

出　　处：《临床荟萃》2009年第12期1033-1035,共3页Clinical Focus

基　　金：吉林省科技厅科技发展计划项目(200705402);吉林省卫生厅科研项目(20082082);北华大学科研基金资助(2008129)

摘　　要：目的研究乙型肝炎病毒感染者CD8+T淋巴细胞T细胞抗原受体(TCR)Vβ基因互补决定区3(CDR3)克隆化特征。方法选用20例乙型肝炎感染者及20例同期健康体检者,应用多引物聚合酶链反应(PCR)方法同时扩增TCR Vβ基因CDR3区多个片段,高分辨率琼脂糖凝胶电泳检测克隆化特征。结果乙型肝炎病毒感染者CD8+T细胞TCR Vβ9和Vβ14克隆化明显高于正常对照组,分别为70.0%vs 25.0%、85.0%vs 30.0%(P<0.01)。结论乙型肝炎病毒感染者存在CD8+T细胞TCR Vβ9和Vβ14克隆性变化。Objective To explore the cloning characteristics of the complementary determining region（CDR）3 distinct of T cell antigen receptor（TCR） Vβ gene of CD8^＋ T cell of HBV inflected persons. Methods The enrollment included 20 cases of HBV infected persons and 20 normal persons from body check at the same time. Polymerase chain reaction assay of poly-primer was applied and meanwhile the many pieces of the CDR3-distinct of the TCR Vβ gene were amplified. HR-agarose gel electrophoresis was employed to detect the cloning characteristics. Results The HBV inflected persons＇ coloning of the TCR Vβ9 and Vβ14 of the CD8^＋ T cell was obviously higher than that of the normal controls, 70. 0% vs 25.0%,85. 0 % vs 30. 0% （ P 〈0. 01）. Conclusion The HB V inflected persons have the cloning changes of the TCR Vβ9 and Vβ14 of the CD8^＋ T cell.

关 键 词：肝炎病毒 乙型 受体 抗原 T细胞 克隆化 

分 类 号：R512.62[医药卫生—内科学]