溃结安康汤对溃疡性结肠炎大鼠结肠组织IL-10的作用及蛋白表达的影响  

The Action to IL-10 and the Influence to Protein Expression of Kuijieankang Decoction to Rats' with Ulcerative Colitis

作　　者：陶弘武[1] 柳越冬[2] 李开明[3] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属第三医院,辽宁沈阳110003 [3]辽宁中医药大学职业技术学院,辽宁沈阳110101

出　　处：《中华中医药学刊》2009年第6期1257-1259,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然基金资助项目(20072072)

摘　　要：目的:研究溃结安康汤对溃疡性结肠炎大鼠白介素(IL)影响及其作用机制。方法:健康W istar大鼠,随机分为空白对照组、模型组、补脾益肠丸组、SASP(柳氮磺胺吡啶)组、溃结安康汤组。TNBS乙/醇法建立溃疡性结肠炎大鼠模型。分组灌胃治疗后,检测溃结大鼠结肠组织IL-10含量和IL-10蛋白表达。结果:模型组与正常组比较,IL-10含量降低、蛋白表达降低,具有统计学意义(P<0.05);补脾益肠丸组、SASP组、溃结安康汤组与模型组比较IL-10含量升高、蛋白表达升高,具有统计学意义(P<0.05);溃结安康汤组与补脾益肠丸组、SASP组比较,IL-10含量升高明显、蛋白表达升高明显,具有统计学意义(P<0.05)。结论:溃结安康汤可以增加溃疡性结肠炎大鼠模型中结肠组织IL-10含量和蛋白表达。Objective ： To research into the influence of Kuijieankang decoction to ratsinterleukin with ulcerative colitis and its mechanism of action. Methods： Some healthy Wistar rats were randomly divided into control group, model group, Bupiyichang pill group, SASP group, and Kuijieankang decoction group. The rat model of ulcerative colitis was established by TNBS/ethanol method. Detecting the IL - 10 content and protein expression of rats＇colon tissue with ulcerative colitis after intragastric administration. Results： Comparing with normal group, the IL- 10 content and protein expression of model group rats descended. It was statistically significant（ P 〈 0.05 ）. The IL - 10 content and protein expression of Bupiyichang pill group, SASP group, Kuijieankang decoction group rose compared to model group. Also was statistically significant（ P 〈 0.05 ）. Comparing Kuijieankang decoction group with Bupiyichang pill group and SASP group ,the IL- 10 content and protein expression of the former rose significantly, with statistical significance （ P 〈 O. 05 ）. Conclusion： Kuijieankang decoction could heighten the IL - 10 content and protein expression of rats＇colon tissue with ulcerative colitis.

关 键 词：溃结安康汤 溃疡性结肠炎 IL-10蛋白表达 

分 类 号：R285.5[医药卫生—中药学]