清毒栓对宫颈癌SiHa细胞及PTEN-MDM2-p53网络环路的影响  

Effect of Qingdushuan on PTEN-MDM2-p53 Network of Cervical Cancer SiHa Cell

作　　者：于妍妍[1] 金哲[2] 楼姣英[2] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《中国中医药信息杂志》2009年第6期33-35,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市自然科学基金(7062038)

摘　　要：目的从细胞生物学及分子生物学水平探讨清毒栓对宫颈癌SiHa细胞增殖抑制及对PTEN-MDM2-p53网络中各个蛋白表达的影响。方法以4%含药血清培养液作用于SiHa细胞,设立正常对照组、空白血清组、清毒栓组、保妇康栓组及干扰素组,通过MTT法检测含药血清对细胞增殖抑制的影响;同时运用Westernblot法检测PTEN-MDM2-p53蛋白的表达情况。结果经含药血清培养液作用后各组细胞数量减少,其中以4%浓度含药血清作用72h时对细胞抑制作用最强,与空白血清组比较,差异有统计学意义(P<0.01);同时,含药血清可升高PTEN、P53抑癌蛋白的表达,降低癌蛋白MDM2的表达。结论清毒栓含药血清可能是通过调控PTEN-MDM2-P53网络环路而达到抑制肿瘤的目的。Objective To discuss the effect of Qingdushuan on SiHa cells of cervical cancer proliferation and protein expression in PTEN-MDM2-p53 network from the level of cell biology and molecular biology. Methods SiHa cells were cultivated with 4% containing serum. Normal control group, blank serum group, Qingdushuan group, Baofukang group and interferon group were established. The influence of inhibiting and proliferating SiHa cell was detected by MTT assay. Three protein expression was detected by Westem blot method. Results After cultured with drug containing serum, the number of cells decreased, 4% concentration of serum for 72 h was the strongest. Compared with the control group, there were significant differences （P〈0.01）. At the same time, drug containing serum elevated expression of tumor suppressor protein of PTEN and p53, reduced cancer protein expression of MDM2. Conclusion Qingdushuan containing serum can inhibit tumor through regulating PTEN-MDM2-D53 network.

关 键 词：清毒栓 宫颈癌 SIHA细胞 PTEN-MDM2-p53网络 

分 类 号：R285.5[医药卫生—中药学]