毒热平注射液对流感病毒FM1感染小鼠免疫功能的影响  

Effect of Dureping Injection on Immunological Function of FM1 Infected Mice

作　　者：景姗[1] 顾立刚[1] 周芸[1] 李澎涛[1] 

机构地区：[1]北京中医药大学中医药抗病毒教育部重点实验室,北京100029

出　　处：《中国中医药信息杂志》2009年第6期37-39,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30772872)

摘　　要：目的研究毒热平注射液对流感病毒FM1感染性肺炎小鼠肺指数、白细胞介素4(IL-4)、干扰素-γ(IFN-γ)基因转录水平、CD40L蛋白表达水平的影响,探讨其抗病毒的作用机制。方法以ICR小鼠(SPF级)作为研究对象。实验分为7组:毒热平注射液大、中、小剂量组及双黄连组、利巴韦林组、FM1感染模型组、正常组。流感病毒FM1感染小鼠造模连续7d后摘取肺组织,计算肺指数,采用RT-PCR法测定肺组织中INF-γmRNA、IL-4mRNA基因转录水平,采用Westernblot法检测肺组织中CD40L蛋白表达水平。结果FM1感染模型组的肺指数明显高于正常组(P<0.001),利巴韦林组、双黄连组及毒热平注射液大、中、小剂量组与FM1感染模型组比较,肺指数均明显降低(P<0.001或P<0.01),其中以毒热平中剂量组最好。毒热平注射液能降低FM1感染小鼠肺组织中IL-4mRNA基因转录(P<0.001),且与浓度成反向关系,但毒热平注射液组的IL-4mRNA基因转录水平明显高于利巴韦林组(P<0.001);毒热平注射液能增强FM1感染小鼠肺组织中IFN-γmRNA基因转录(P<0.001),且与浓度呈正向相关性,毒热平注射液大、中剂量组与利巴韦林组比较无统计学意义(P>0.05)。毒热平注射液能抑制FM1感染小鼠肺组织中CD40L蛋白表达(P<0.001),抑制效果与浓度呈正相关性,但毒热平注射液中、小剂量组CD40L蛋白表达水平显著高于正常组与利巴韦林组(P<0.05)。结论毒热平注射液体内能降低流感病毒FM1感染小鼠的肺指数,增强肺组织IFN-γmRNA基因转录,抑制IL-4mRNA基因转录,抑制流感病毒FM1感染小鼠肺组织CD40L蛋白的表达。Objective To investigate the effect of Dureping injection （DRP） on IL-4, IFN-γ mRNA and CD40L protein expression of FM 1 infected mice with virus pneumonia, and explore the mechanism of antiviral effect. Methods ICR mice were divided into 7 groups： DRP1, DRIP, DRP3, SHL, Ribavirin, sham and model group. The mice were sacrificed on the 7th after infected, and the lung index was calculated. Then, IFN-γ mRNA and IL-10 mRNA in the lung tissue was measured with RT-PCR, CD40L protein in the lung tissue was measured with Western-blot. Results The lung index of mice in model group was significantly higher than sham group （P〈0.001）. Compared with model group, the lung index of mice in Ribarivin, SHL, DRP1, DRP2 and DRP3 groups was significantly decreased （P〈0.001 or P〈0.01）, and the function of DRP2 was the best. Compared with model group, DRP showed down-regulation on the transcription of IL-4 in FM 1 infected mice lung （P 〈0.001）, and up-regulation on the transcription of INF-γ in FM1 infected mice lung （P〈0.001）. The expression of CD40L protein in Ribarivin, SHL, DRP1, DRP2 and DRP3 was significantly decreased compared with model group （P〈0.001）, but the expression of CD40L protein in DRP2, DRP3 groups was significant highter than sham group and Ribarivin group （P〉0.05）. Conclusion DRP in vivo could down-regulate the lung index, inhibit the transcription IL-4 mRNA, enhance the transcription of IFN-γ and inhibit the expression of CD40L protein in lung of FM 1 infected mice.

关 键 词：毒热平注射液 流感病毒FM1 白细胞介素4 干扰素-Γ CD40L 小鼠 

分 类 号：R285.5[医药卫生—中药学]