复合酶辅助超声波提取沙枣多糖的工艺研究  

Study on Extraction Process of Polysaccharides from Elaeagnus angustifolia L. by Enzymatic- assisted Ultrasonic Methods

作　　者：廉宜君[1] 李炳奇[1] 刘红[1] 马彦梅[1] 陈韩英[2] 

机构地区：[1]石河子大学化学化工学院,新疆石河子832000 [2]石河子大学药学院,新疆石河子832000

出　　处：《中国中医药信息杂志》2009年第6期47-48,59,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：石河子大学高层次人才科研启动资金专项(RCZX200713)

摘　　要：目的研究复合酶辅助超声波提取沙枣多糖的最佳工艺。方法考察了纤维素酶和果胶酶在不同温度、不同酶量、不同酶作用时间下提取沙枣多糖最佳的方案,并且研究了超声处理对于酶法提取沙枣多糖的影响。结果沙枣多糖提取的适宜条件是:纤维素酶1.5%、果胶酶1.5%;酶解时间为40min,酶解温度为55℃,超声时间25min,超声功率400W,固液比1∶30,超声温度60℃。在此条件下,沙枣多糖的提取率可达12.35%。结论本工艺简单可行,可用于沙枣多糖的提取。Objective To optimize the technological conditions of polysaccharide extraction from Elaeagnus angustifolia L. by enzymatic-assisted ultrasonic. Methods The effects of different enzymes, enzymolysis temperature, enzyme dosage, enzymolysis time, material-water ratio, crushing degree, ultrasonic time and ultrasonic temperature on the extraction rate of Elaeagnus angustifolia L. polysaccharide was analyzed. Results The optimal technological condition was as follows： 1.5% cellulase, 1.5% pectic enzyme, enzymolysis time 40 min, enzymolysis temperature 55℃, ultrasonic time 25 min, ultrasonic power 400 W, material- water ratio 1 ： 30, and temperature 60 ℃. In these conditions, the extraction efficiency of polysaccharide was 12.35%. Conclusion The process is simple and practicable, and can be used for the extraction of Elaeagnus angustifolia L. polysaccharide.

关 键 词：沙枣 多糖 酶 超声波 提取工艺 

分 类 号：R283.5[医药卫生—中药学]