桂枝茯苓丸对荷瘤鼠细胞凋亡及相关基因表达的影响  

Study on GFW Affecting Tumor Apoptosis in Bearing Tumor Mice and the Related Genes Expression

作　　者：张丽宏[1] 王琪[2] 颜培宇[1] 王亚贤[1] 

机构地区：[1]黑龙江中医药大学微生物学与免疫学教研室,黑龙江哈尔滨150040 [2]齐齐哈尔医学院免疫学教研室,黑龙江齐齐哈尔161042

出　　处：《辽宁中医杂志》2009年第6期1032-1034,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：黑龙江省教育厅资助课题(11511458)

摘　　要：目的:探讨桂枝茯苓丸诱导肿瘤细胞凋亡机制,为桂枝茯苓丸(GFW)开发应用提供实验依据。方法:计算抑瘤率,流式细胞仪测定细胞凋亡率,免疫组化法测定P21waf/cip蛋白表达,原位杂交法检测Survivin mRNA表达,电镜观察肿瘤细胞超微结构。结果:GFW抑瘤率为38.93%,流式细胞仪检测GFW组凋亡率17.79%,与模型组比较,差异有统计学意义(P<0.05)。GFW上调肿瘤细胞P21waf/cip蛋白表达,下调Survivin mRNA表达。GFW组镜下可见瘤细胞以凋亡变化为主。内膜结构完好,核膜清晰,细胞核固缩,染色质团块状散布核内或边集核膜下,并可见凋亡小体。结论:GFW诱导肿瘤细胞凋亡,其机制可能与上调P21及下调Survivin mRNA表达密切相关。Objective：To Study GFW on tumor apoptosis and to apply the experimenting basis of GFW, s developing and utilizing. Methods：The S180 mice sarooma model was used to detective the inhibiting effects of GFW, observed the ultrastmctural change by electron microscopy and the flow cytometer was used for determination apoptosis. The protein expression of P21 and the mRNA expression of Survivin were evaluated by immunohistochemistry and in situ hybridization. Resu/ts ： For S180 sarcoma, the inhibiting ratio of GFW is 38.93%. The apoptosis isl7. 79% by the flow cytometer. Respectively,some typical apoptotic cells and apoptotie bodies were observed through electron microscope. Chromatin gathered side of cell, the nucleus turns into the nucleus band and nucleus project. Survivin mRNA was markedly declined in the GFW group when compared with the model group（ P 〈 0. 01 ） ,while the postitive expression of P21^waf/cip was obviously higher in theGWF group than in the model group （ P 〈 0.01 ）. Conclusion：GFW induces the tumor cell apoptosising, its mechanism maybe closely related with adjusting expression levels of P21^waf/cip and Survivin gene.

关 键 词：桂枝茯苓丸 P21^waf/cip 生存素 凋亡 

分 类 号：R285.5[医药卫生—中药学]