^(137)Cs γ辐照和NaN_3处理对毛竹种子发芽率和保护酶活性的影响  

EFFECTS OF ^(137)Cs γ-RAYS IRRADIATION AND NaN_3 TREATMENT ON GERMINATION RATE AND PROTECTIVE ENZYME ACTIVITY FOR SEEDS OF Phyllostachys heterocycla cv. Pubescens

作　　者：桂仁意[1] 刘亚迪[1] 郭小勤[1] 方伟[1] 

机构地区：[1]浙江林学院浙江省现代森林培育技术重点实验室,浙江临安311300

出　　处：《核农学报》2009年第3期400-404,412,共6页Journal of Nuclear Agricultural Sciences

基　　金：浙江省重大科技攻关项目(2005C12002-01)

摘　　要：用不同剂量的137Csγ射线和不同浓度的NaN3对毛竹种子进行诱变处理,研究2种诱变方式对毛竹种子发芽率以及萌发种子内保护酶活性的影响,以期为开展毛竹诱变育种工作奠定理论基础。结果表明:10Gy剂量的γ射线辐照促进种子萌发,随着剂量的增加,对种子的发芽率抑制作用达极显著性水平,而NaN3处理对种子的发芽率的抑制则达显著水平,说明137Csγ射线对毛竹种子产生的诱变效应较NaN3明显;不同剂量γ射线和不同浓度NaN3对萌发种子中保护酶活性的影响均达到极显著水平,并且在低剂量和高剂量时均出现明显的变化临界点,分析得出保护酶活性可以反映毛竹种子对γ射线和NaN3的敏感性。初步选择出137Csγ射线和NaN3对毛子种子的半致死剂量和浓度分别为95.5Gy和0.16mmol/L,可以将其作为毛竹种子适宜的诱变剂量。The seeds of Phyllostachys heterocycla cv. Pubescens were treated by using ^137 Cs γ-rays irradiation and NAN3, and the effects of two mutation methods on the germination rates and protective enzyme activities in the germinating seeds were investigated, and set up a theoretical foundation for mutation breeding of bamboo. The results showed that 10Gy irradiation could promote seed germination. As the dose increased, seed germination rate was significantly inhibited in the case of NaN3 treatment. This showed that a mutagenic effect of γ-rays was more obvious than that of NaN3. The effects of different doses of γ-rays and different concentrations of NaN3 on the protective enzyme activity were extremely significant. And the obvious changing point occurred at both low and high doses （concentrations）. The protective enzyme activity could reflect the sensitivity. The LD50 for the ^137 Cs γ-rays radiation and NaN3 concentration were 95.5Gy and 0.16 mmol / L, respectively, which could be considered as the optimum dose （concentration） for mutation breeding of bamboo.

关 键 词：毛竹 137Csγ射线辐照 NAN3 发芽率 保护酶活性 

分 类 号：S795[农业科学—林木遗传育种]