体外培养子宫腺肌病在位内膜腺上皮中Kiss-1和乙酰肝素酶的表达及意义  

The expressions of Kiss-1 and heparanase in cultured glandular cells from eutopic endometrium in adenomyosis

作　　者：张亚琴[1] 李莉[1] 

机构地区：[1]山西医科大学第一医院妇科,太原030001

出　　处：《中国药物与临床》2009年第6期489-491,共3页Chinese Remedies & Clinics

基　　金：山西省科技厅攻关项目(042024-2)

摘　　要：目的探讨Kiss-1和乙酰肝素酶(Hpa)在子宫腺肌病发生、发展中的作用。方法选择10例子宫腺肌病患者作为研究组,8例宫颈上皮内瘤变(CIN)Ⅲ级患者作为对照组,通过对子宫内膜腺上皮细胞的体外培养,用反转录-聚合酶链反应半定量分析Kiss-1和Hpa mRNA在研究组、对照组子宫内膜腺上皮细胞的表达。结果研究组腺上皮细胞Kiss-1 mRNA的表达在增殖期与分泌期均较对照组降低,差异有统计学意义(P<0.05),而同一组中增殖期腺上皮细胞Kiss-1 mRNA的表达与分泌期相比差异无统计学意义(P>0.05)。研究组腺上皮细胞Hpa mRNA的表达在增殖期与分泌期均较对照组升高,差异有统计学意义(P<0.05),而同一组中增殖期腺上皮细胞Hpa mRNA的表达与分泌期相比差异无统计学意义(P>0.05)。结论Kiss-1 mRNA表达减弱和Hpa mRNA表达增强可能与子宫腺肌病的侵袭过程有关,两者在腺肌病的发生发展中发挥重要作用。Objective To investigate the relationship between Kiss-1 and heparanase expressions and the development and progression of adenomyosis. Methods The expressions of Kiss-1 and Hpa mRNA in cultured glandular cells in adenomyosis group （n=10） and control group （8 patients with CIN Ⅲ） were detected by semi-quantitative RT-PCR. Results The level of Kiss-i mRNA expression in the glandular cells in adenomyosis group was obviously decreased compared with control group during both proliferative and secretory phases （P〈0.05）, but no difference was observed during secretory phase compared with proliferative phase in the same group （P〉0.05）. The expression of Hpa mRNA in the glandular cells in adenomyosis group was obviously increased compared with control group during both proliferative and secretory phase （P〈0.05）, but the level of Hpa mRNA expression in the glandular cells did not differ during secretory phase compared with proliferative phase in the same group （P〉0.05）. Conclusion The decreased expression of Kiss-1 mRNA and the increased expression of Hpa mRNA may be involved in the invasion of adenomyosis and may play an important role in the development and progression of adenomyosis.

关 键 词：子宫腺肌病 乙酰肝素酶 KISS-1 

分 类 号：R711.71[医药卫生—妇产科学]