苦皮藤毒杀成分对昆虫的选择毒杀作用及其机制研究  

Studies on the Selective Toxicity and Selective Mechanism of Poisonous Ingredient Celangulin Ⅴ of Celastrus angulatus Against the Insect Pests

作　　者：刘惠霞[1] 吴文君[1] 姬志勤[1] 王金丽 王美珍[1] 

机构地区：[1]西北农业大学植物保护系

出　　处：《西北农业学报》1998年第2期41-44,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金

摘　　要：生测结果表明，苦皮藤毒杀成分苦皮藤素Ⅴ若被试虫摄入消化道（胃毒处理），则在小地老虎Ａｇｒｏｔｉｓｙｐｓｉｌｏｎ和粘虫Ｍｙｔｈｉｍｎａｓｅｐａｒａｔａ幼虫之间有明显的选择毒杀作用：对小地老虎４龄幼虫，即使给予高达１０５ｍｇ／ｇ的剂量，试虫也不表现任何中毒症状，而对粘虫４龄幼虫，仅给予３７６μｇ／ｇ的剂量，试虫就表现失水、死亡。若直接将苦皮藤素Ⅴ注入试虫血淋巴，则小地老虎和粘虫４龄幼虫都表现相同的失水、死亡症状，不表现选择毒杀作用。这种胃毒选择毒杀作用的机理包括２个方面：①药剂在虫体内穿透作用的差异。苦皮藤素Ⅴ很容易从粘虫幼虫消化道穿透肠壁进入血腔，也容易从血腔进入消化道；但苦皮藤素Ⅴ既不能从小地老虎幼虫消化道进入血腔，也不能从血腔进入消化道；②试虫解毒能力的差异。活体试验和离体试验都说明小地老虎幼虫对苦皮藤素Ⅴ的解毒速率远大于粘虫幼虫。The bioassay results of the poisonous ingredient celangulin Ⅴ against the 4thinstar larvae of Mythimna separata and Agrotis ypsilon with oral applicating method showed that celangulin Ⅴ had selective poisonous action:the larvae of Mythimna separata could lose the bodyliguid and fall into death at a dosage of 376 μg/g, no individual of the larva of Agrotis ypsilon would be exhibited any poisonous symptom, even though at a dosage of 105 mg/g The bioassay results of celangulin Ⅴ agaist the 4thinstar larvae of Mythimna separata and Agrotis ypsilon  with injection applicating method showed that celangulin Ⅴ had no selective poisonous action, both Mythimna separata and Agrotis ypsilon  larvae could lose the bodyliquid and fall into deathTwo respects of the mechanism might be involved in this selective toxicity:1The difference of penetrationIn the larval body of Mythimna separata, celangulin Ⅴ could penetrate the midgut wall from the digestive tube into the haemocoele, or from haemocoel into the digestive tube, while in the larval body of Agrotis ypsilon, celangulin Ⅴ could not penetrate the midgut wall neither from the digestive tube into the haemocoele, nor from the hacmocoele into the digestive tube2The difference of detoxicityThe testing results in　Vivo or in Vitro indicated that the rate of degradation and detoxicity of celangulin Ⅴ in the larval body of Agrotis ypsilon was higher than in the larval bdy ofMythimna separata

关 键 词：苦皮藤 苦皮藤素V 选择毒性 药效试验 

分 类 号：S482.3[农业科学—农药学] S481.9[农业科学—植物保护]