小鼠胚胎干细胞无饲养层培养及其生物学特性  

Culture of Mouse Embryonic Stem Cells and Their Biological Features Without Feeder Layer Cells

作　　者：韩甫[1] 叶荣[1] 鲍柳君[1] 张军超[1] 张焕相[1] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院细胞生物学系江苏省干细胞研究重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2009年第2期204-206,F0003,共4页Suzhou University Journal of Medical Science

基　　金：973项目资助(2005CB623906);江苏省教育厅重大项目(06KJA18025)

摘　　要：目的建立小鼠胚胎干(ES)细胞株S8在无饲养层的条件下短期内连续传代体系,获得大量纯的ES细胞。方法ES-S8细胞无饲养层连续传代后,检测其未分化指标以及多分化潜能。结果ES-S8细胞在无饲养层条件下培养5代,AKP染色、SSEA-1免疫荧光、OCT-4均显阳性,以及经拟胚体(EB)途径自发分化后,能形成多种类型的细胞。结论ES-S8细胞能够在无饲养层条件下培养,至少传5代仍能保持未分化特异性指标和多分化潜能。Objective To establish the method of short-term cultures of mouse embryonic stem cells S8 without feeder layer cells and obtain robust pure ES-S8 cells. Methods ES-S8 cells were cultured for five passages without feeders. The undifferentiated state was confirmed by expression of alkaline phosphatase,SSEA-1 and Oct-4, and the pluripotency of EBs analysed. Results ES-S8 cells were positive for AKP, SSEA-1 and Oct-4 without feeders, and differentiated into a variety of cell types. Conclusion ES-S8 can grow normally at least 5 passages without feeders, and maintain their undifferentiated characteristics and pluripotency.

关 键 词：胚胎干细胞 饲养层细胞 拟胚体 小鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] Q813.11[医药卫生—基础医学]