硫酸镁对大鼠急性放射性脑损伤后脑NMDA受体及血清神经元特异性稀醇化酶的影响  

Effect of MgSO_4 on NMDA Receptor in Brain Tissue and Serum NSE in Rats with Radiation-induced Acute Brain Injury

作　　者：袁文佳[1] 崔凤梅[1] 王利利[2] 刘萍[1] 何超[1] 涂彧[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院 [2]苏州大学附属第一医院肿瘤放疗科,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2009年第2期224-226,231,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30670638)

摘　　要：目的探讨硫酸镁对电离辐射诱发的脑组织损伤的保护作用。方法将成熟的SD大鼠36只随机分为空白对照组、实验对照组和硫酸镁实验用药组,用6 MeV电子线对实验组大鼠进行20 Gy全脑单次垂直照射,吸收剂量率为200 cGy/min,分别于照射后1、3、7和14 d解剖大鼠行腹主动脉取血,取其脑组织,用酶联免疫吸附法(ELISA)测定血清神经元特异性稀醇化酶(NSE)含量,Western blot方法检测大鼠脑组织N-甲基-D-天门冬氨酸受体NR1及NR2B蛋白表达量的变化。结果与空白对照组相比,实验对照组大鼠血清NSE含量及脑组织NR1和NR2B蛋白表达量升高,各时间点血清NSE含量差异均有统计学意义(P<0.05);实验用药组与实验对照组相比,照射后1d血清NSE含量差异没有统计学意义(P>0.05),但随着照射后观察时间的延长,NSE含量出现显著降低,差异有统计学意义(P<0.05),直到14d仍然未恢复至正常水平;实验用药组大鼠脑组织NR1和NR2B蛋白表达量在照射后的各时间点与实验对照组相比,均有所降低。结论早期使用硫酸镁可降低照射后血清NSE的含量及脑组织NR1和NR2B蛋白表达量,硫酸镁对急性放射性脑损伤有保护作用。Objective To explore the protection of magnesium sulfate（MgSO4） on radiation-induced acute brain injury. Methods Thirty six mature Sprague-Dawley rats were randomly divided into 3 groups：the blank control group, experimental control group and experimental therapy group.The whole brain of SD rats in the experimental control group and experimental therapy group were irradiated with a dose of 20 Gy using 6 MeV electron beam.Magnesium sulfate was injected intraperitoneally into the rats in the experimental therapy group before and after irradiation for seven times.The blood and the brain tissue were taken on the 1st, 3rd and 14th day after irradiation.ELISA was used to measure the level of serum NSE.Westeru blot technique was used to detect the expression of NR1 and NR2B subunit protein in brain tissue. Results Compared with the blank control group, the level of serum NSE in the experimental control group increased significantly （P〈0.05）. The expression of NR1 and NR2B also increased. Compared with that in the experimental control group, the level of serum NSE in the experimental therapy group was significantly lower since the 3rd day after irradiation （P〈0.05）.The expression of NR1 and NR2B was lower after irradiation. Conclusions MgS04 used in early stage can inhibit the level of serum NSE and the expression of NR1 and NR2B after radiation-induced acute brain injury. It shows a protective effect on radiation-induced acute brain injury.

关 键 词：放射性脑损伤 硫酸镁 神经元特异性稀醇化酶 NMDA受体 

分 类 号：R811.74[医药卫生—放射医学]