高致病性猪繁殖与呼吸综合征病毒分子流行病学调查  

Molecular epidemiology of highly pathogenic porcine reproductive and respiratory syndrome virus in China

作　　者：侯丽丽[1,2] 赵铁柱[2] 遇秀玲[2] 曹振[2] 邓小雨[2] 乔明明[2] 张硕[2] 王斌[2] 陈西钊[2] 田克恭[2] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]中国动物疫病预防控制中心,北京100094

出　　处：《中国兽医学报》2009年第6期677-682,共6页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑计划资助项目(2007BAD86D03;2006BAD06A01)

摘　　要：2006年6月份以来,我国多个省份暴发高致病性猪繁殖与呼吸综合征,给我国养猪业造成了巨大经济损失。为全面了解高致病性猪繁殖与呼吸综合征病毒在我国的流行情况以及遗传变异规律,本研究采用RT-PCR的方法,对从2006年8月至2007年10月期间,来自于19省(市)共474份样品进行高致病猪繁殖与呼吸综合征病毒核酸的检测,结果检出阳性样品294份。在各省不同时期样品中均有阳性样品检出。通过对阳性样品进行Nsp2基因和ORF5基因扩增,序列分析发现所有检测的猪繁殖与呼吸综合征病毒株高度同源,为同一毒株遗传变异而来,所有猪繁殖与呼吸综合征毒株与HB-1株遗传关系最近;且Nsp2均缺失30个氨基酸。In the summer of 2006, a large-scale outbreak of highly pathogenic PRRS in China was reported. To fully understand the knowledge of highly pathogenic PRRSV epidemiology and the extent of genetic diversity in China,we analysed 474 samples obtained from 19 provinces using RT-PCR assay. 294 samples of them were positive. Subsequent sequencing of the PCR products from Nsp2 and ORF5 and phylogenetic analysis revealed that all positive strains are highly homologous to each other, may have evolved from the same ancestor. Moreover, they were most similar to HB-1. At the same time, we observed a unique molecular hallmark in these viral isolates, a discontinuous deletion of 30 amino acids in nonstructural protein 2 （Nsp2）.

关 键 词：高致病性猪繁殖与呼吸综合征病毒 NSP2 ORF5 

分 类 号：S852.65[农业科学—基础兽医学]