外加碳源对优良菌降解芘的影响研究  

INFLUENCE OF EXOTIC CARBON SOURCE ON PYRENE BIODEGRADATION BY PREDOMINANT STRAINS

作　　者：王蕾[1] 聂麦茜[1] 王志盈[1] 惠艳[1] 张敏娜[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《水处理技术》2009年第6期24-27,31,共5页Technology of Water Treatment

基　　金：陕西省教育厅自然专项项目(07JK290)

摘　　要：通过溶剂萃取,薄层色谱,紫外分光光度法以及MPN计数等方法研究了外加碳源对一株放线菌CB2#和一株细菌CB9#降解芘的影响特性。结果表明,具有较高菌体表面疏水性的CB2#菌对芘的降解率比亲水性的CB9#菌对芘的降解率高。对于CB2#菌,通过外加邻苯二甲酸和葡萄糖其对芘的降解效率可分别提高25%和16%;对于CB9#菌,外加邻苯二甲酸和葡萄糖后,其菌体表面对芘表现出很强的吸附性,且其生物降解过程受到明显的抑制;在以上降解液中pH随时间在小范围内波动,说明CB2#菌和CB9#菌降解芘的过程是周期性的交替出现产酸和脱羧的动态过程。The influence of exotic carbon source on pyrene biodegradation by a bacteria and an actinomycete was investigated by designed solvent exaction experiments, thin layer chromatography （TLC）, UV-spectrophotometry analysis and MPN method. The results indicated that the biodegradation rate was higher by CB^# with higher hydrophobicity than by CB9^# with higher hydrophilicity. For CB2^#, the pyrene degradation rate can be enhanced 25% and 16% by adding phthalic acid and glucose respectively. For CB9^#, it displayed high adsorptivity to pyrene, and its pyrene-degrading process could be inhibited by adding phthalic acid and glucose in cultural liquid. The pH values of biodegrading medium were fluctuating up and down continuously, this pH value fluctuations were resulted from accumulating of acidic metabolic intermediates and further decarboxylation of such intermediates.

关 键 词：芘 优良菌 外加碳源 PH 降解率 

分 类 号：X703[环境科学与工程—环境工程]