冰岛刺参调节血脂及其作用机制  

Blood Lipid Regulation Effects and Mechanisms of Cucumaria frondosa on Experimental Hypercholesterolemia Rats

作　　者：高森[1] 王静凤[1] 王玉明[1] 刘治东[1] 李晓林[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《武汉大学学报（理学版）》2009年第3期324-328,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家高技术研究发展计划(863)项目(2007AA091805);国家科技支撑计划项目(2008BAD94B05);国家自然科学基金资助项目(30871944)

摘　　要：本实验研究了冰岛刺参(Cucumaria frondosa)对实验性高胆固醇血症大鼠脂质代谢的调节作用及其机制.采用灌胃高脂乳剂方法建立实验性高胆固醇血症大鼠模型,检测血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)含量;肝脏TC、TG含量和肉毒碱棕榈酰转移酶-1(carnitine palmitoyltransterase-1,CPT-1)、过氧化物酶体β-氧化酶系(peroxisomal-βoxidation enzymes,POE)、磷脂酸磷酸酶(Phospha-tidic acid phophyhydrolase,PAP)活力;粪便总脂质、TC和胆汁酸排出量.实验结果显示,冰岛刺参能显著降低高胆固醇血症大鼠血清TC(p<0.05)、LDL-C(p<0.05)含量及LDL-C/HDL-C(p<0.05);显著降低肝脏TC(p<0.01)、TG(p<0.01)含量,提高CPT-1(p<0.01)和POE(p<0.05)活力;显著提高粪便总脂质(p<0.01)、胆固醇(p<0.01)和胆汁酸(p<0.01)的排出量.冰岛刺参能有效调节高胆固醇血症大鼠的脂质代谢,其作用机制主要是促进脂质在体内的氧化和异化,同时抑制其在消化道内的吸收.The investigation on the hypolipidemic effect and the mechanism of C. frondosa on experimental hypercholesterolemia ras were conducted in this experiment. The model for the rats with hypercholesteremia was established by administering them orally with high fat emulsion. The following indexes were examined, such as the contents of TC, TG, LDL-C and HDL-C in serum; the contents of TC and TG, the activities of carnitine palmitoyl transterase-1 （CPT-1）, peroxisomal β-oxidation enzymes （POE） and phosphatidic acid phophyhydrolase （PAP） in liver; the excurrent contents of total lipids, TC and bile acid in fecal. The results showed that C. frondosa could decrease the contents of TC （p〈0.05）, LDL-C （p〈0.05） and the LDL-C/HDL-C （p〈0. 05） in serum significantly; reduce the contents of TC （p〈0.01） and TG （p〈0.01）, and enhance the activities of CPT-1 （p〈0.01） and POE （p〈0.05） of liver markedly; increase the contents of total lipids （p〈0.01）, TC （p〈0.01） and bile acid （p〈0.01） in fecal effectively. It can be concluded that C. frondosa can alleviate the disturbance of lipid metabolism induced by hypercholesteremia, The mechanisms are related to enhancing the oxidation and dissimilation of lipids and inhibiting the absorption of lipids.

关 键 词：刺参 肉毒碱棕榈酰转移酶-1 过氧化物酶体β-氧化酶系 磷脂酸磷酸酶 胆汁酸 

分 类 号：Q493.5[生物学—生理学]