给药硝酸钕后大鼠完整肝组织及肝组织提取物的NMR代谢组学研究  

Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate

作　　者：廖沛球[1,2] 魏来[1,2] 刘威[3] 吴亦洁[1] 李晓晶[1] 倪嘉缵[1] 裴奉奎[1] 

机构地区：[1]中国科学院长春应用化学研究所,长春130022 [2]中国科学院研究生院,北京100049 [3]吉林省中医药科学院,长春130021

出　　处：《高等学校化学学报》2009年第6期1116-1120,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20575065和20637010)资助

摘　　要：通过分析不同给药剂量硝酸钕[Nd(NO3)3][2,10,50 mg/kg(体重)]后,雄性Wistar大鼠完整肝组织的MAS1HNMR谱和肝组织提取物的1HNMR谱,结合肝组织病理切片图,研究了稀土化合物Nd(NO3)3在大鼠体内的急性生物效应.利用模式识别方法对给药Nd(NO3)3组和对照组大鼠肝组织1H NMR谱图数据进行了分析.结果表明,腹腔注射Nd(NO3)3后,大鼠肝脏中甘油三酯、亮氨酸(异亮氨酸)、乳酸、丙氨酸、丙酮酸、磷酸胆碱和葡萄糖含量升高,氮氧三甲胺含量降低.肝脏病理图显示,50 mg/kg(体重)组大鼠肝细胞可见微小坏死灶和门管区炎细胞轻度增多.推测硝酸钕能影响大鼠肝脏中能量代谢(糖代谢和脂肪代谢)和氨基酸代谢,对大鼠肝脏造成损伤,且其损伤程度随剂量的增加有增强趋势.The biochemical effects of neodymium nitrate were studied by NMR spectroscopic-based metabonomic approach. Male Wistar rats were treated intraperitoneally with different doses （2, 10 and 50 mg/kg body weight） of Nd（ NO3 ）3. Liver samples from Nd^3＋ -treated rats were analyzed either by 600 MHz ^1H MAS NMR techniques for intact liver or using high resolution （liquid state） ^1H NMR for liver chloroform/water/methanol extracts. All NMR spectra data were analyzed by pattern recognition using principal components analysis. Liver lesions induced by Nd （NO3 ）3 were represented by increased triglyceride, leucine （isoleucine）, lactate, alanine, pyruvate, phosphocholine and glucose concentration, and reduced trimethylamine N-oxide （TMAO） concentration in liver. Histopathology examination showed cell necrosis and inflammation in the liver of 50 mg/kg body weight dosed rats. It was demonstrated that neodymium nitrate could cause damages to rats liver by disturbing the energy metabolism（ glucose and lipid metabolism） and amino acid metabolism in the liver.

关 键 词：核磁共振 代谢组学 模式识别 肝 硝酸钕 生物效应 

分 类 号：O657.2[理学—分析化学]