玻璃微流控通道中水凝胶固定寡核苷酸探针的方法及应用  

Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications

作　　者：张宇[1,2] 于浩[1] 董秀玲[3] 秦建华[1] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院研究生院,北京100049 [3]大连市第三人民医院,大连116033

出　　处：《高等学校化学学报》2009年第6期1128-1130,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20635030,20575067);科技部“八六三”计划(批准号:2006AA02Z305)资助

摘　　要：A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels v/a photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0. 1 μmol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.

关 键 词：微流控芯片 丙烯酰胺水凝胶 DNA探针固定 核酸杂交 

分 类 号：O652.6[理学—分析化学]