栒子试管苗蛭石支撑生根培养及炼苗移栽研究  

Study on the Rooting Culture and Hardened-seedling Transplanting of Cotoneaster hjelmqvistii Plantlets on the Medium Supported by Vermiculite

作　　者：柴慈江[1] 史燕山[1] 骆建霞[1] 卢兴霞[1] 王丹[1] 刘颖[1] 马秋英[1] 

机构地区：[1]天津农学院园艺系,天津300384

出　　处：《安徽农业科学》2009年第17期7860-7861,7871,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技部星火计划项目(2008GA610015);天津市科委项目(08ZHXHNC07000)

摘　　要：[目的]探讨蛭石作培养基支撑物对栒子试管苗生根培养及炼苗移栽的影响。[方法]将栒子微茎置于蛭石支撑的培养基进行生根培养,将培养成的木旬子试管苗经开瓶炼苗2周后带坨移入营养钵中,观察移栽后的成活率,并观察开瓶炼苗期间栒子试管苗叶片气孔在黑暗条件下的开闭状况。[结果]栒子微茎在蛭石支撑的培养基中可形成正常的试管苗,其生根率达到88.3%,显著高于琼脂培养基(66.7%)。对栒子试管苗移栽前进行2周的开瓶炼苗,可使其叶片气孔的关闭功能得到明显恢复。栒子试管苗经炼苗带坨移栽后,在不喷雾、不覆膜及午后空气相对湿度低至60%的条件下成活率达到86.7%,显著高于常规移栽(31.7%)。[结论]该研究为完善栒子组织培养快繁技术提供依据。[ Objective] The study aimed to discuss the effect of Vermiculite used the supporting of medium on rooting culture, acclimatizing and transplanting of Cotoneaster hjelmqvistii test-tube plantlets. [Method] The micro stem of C. hjelmqvistii was put in the vermiculite supporting medium for rooting culture. After acclimatized on opened cultural bottles for two weeks the plantlets rooted on the vermiculite supporting medium were transplanted with a lump of vermiculite into the nutrition pots, and the survival rate of the plantlets was observed, and the stoma closing state of C. hjelmqvistii test-tube plantlets was also observed during opening bottle and acclimatizing the plantlets. [Result] The micro stem of C. hjelmqvistii cultured on the vermiculite supporting medium could get into normal test-tube plantlets and the rooting rate was 88.3%, which was obviously higher than the rooting rate （66.7%） of the micro stem cultured in agar supporting medium. The stoma closing function of C. hjelmqvistii plantlets could be obviously recovered by opening the bottle and acclimatizing the plantlets for two weeks before transplanting. After C. hjelmqvistii test-tube plantlets were acclimatized and transplanted with a lump of vermiculite, the survival rate of the plantlets was 86.7 % under the condition of no-spraying, no-covering and low air humidity of 60% in afternoon, being obvious higher than the survival rate （31.7%） of the plantlets transplanted with normal method. [Conclusion] This study provided the basis for improving the tissue culture propagation techniques of C. hjelmqvistii.

关 键 词：栒子 试管苗 蛭石 生根 移栽 

分 类 号：S603.6[农业科学—园艺学]