NaCl盐胁迫对锦鸡儿保护酶系的影响  

Effect of NaCl Salt Stress on Protective Enzyme System of Caragana sinica

作　　者：石国亮[1] 江萍[1] 

机构地区：[1]石河子大学农学院,新疆石河子832003

出　　处：《安徽农业科学》2009年第17期7963-7965,共3页Journal of Anhui Agricultural Sciences

基　　金：石河子大学自然科学基金项目(5006-822017)

摘　　要：[目的]探索锦鸡儿在盐胁迫下的生化指标。[方法]对锦鸡儿幼苗进行不同浓度的NaCl处理,测定并比较了幼苗体内SOD、CAT和POD活性的变化。[结果]在NaCl胁迫下,锦鸡儿幼苗体内SOD、POD和CAT活性随NaCl浓度的增高均呈先上升后下降的趋势,并可清除幼苗体内的部分活性氧,对幼苗起一定的保护作用。这3种酶活性在NaCl浓度为300 mmol/L时均出现峰值,超过这个临界浓度后均显著下降。锦鸡儿幼苗体内活性氧的保护酶体系主要途径是SOD-POD+CAT。SOD一直保持较高水平,可清除超氧自由基使幼苗初期免受伤害,同时,SOD催化超氧自由基的产物由POD和CAT共同协作来清除,表明3种酶的协同作用增强了植物对逆境的忍耐力。[结论]300 mmol/L的NaCl浓度是适宜锦鸡儿生长的临界土壤盐浓度。[ Objective] The study aimed to discuss the biochemical indexes of Caragana sinica under NaCl sah stress. [ Method] Seedlings of C. sinaca were treated with different coven, of NaCl, and SOD, CAT and POD activity of young seedlings were measured and compared. [ Result ] Under NaCl salt stress, SOD, POD and CAT activity in young seedlings of C. sinica showed a trend of first rise and then fall with the inereaso of NaCl cohen., and could remove some reactive oxygen in seedings, which had a protective effect on seedlings. 3 kinds of enzyme activity had peaks all appeared at NaCl COhen. of 300 mmol/ L, and decreased significantly when NaCl exceeded the critical concn. The main way of protective enzyme system of reactive oxygen species in young seedlings of C. sinica was SOD - PDO ＋ CAT. SOD continuously maintained at a higher level so as to remove the superoxide radical and protect the young seedlings from harm, while SOD catalytic product of supemxide radical was removed by POD and CAT together, which indicated that the synergy of 3 kinds of enzymes enhanced the endurance of plants to stress. [Conclusion] Critical salt concn, suitable for the growth of C. sinica was 300 mmol/L.

关 键 词：锦鸡儿 超氧化物歧化酶 过氧化物酶 过氧化氢酶 

分 类 号：S311[农业科学—作物栽培与耕作技术]