不同提取方法对秦艽中龙胆苦苷提取率的影响  

Study on the Effects of Different Extraction Methods on the Extraction Rate of Gentiopicroside from Gentiana straminea Maxim.

作　　者：张兴旺[1,2] 于瑞涛[1,2] 梅丽娟[1] 邵斌贝 陶燕铎[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院研究生院,北京100049

出　　处：《安徽农业科学》2009年第17期7984-7985,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技攻关项目(0713351611)

摘　　要：[目的]建立RP-HPLC法研究不同提取方法对秦艽中龙胆苦苷提取率的影响，为秦艽药材的合理开发利用提供理论依据。[方法]采用C18色谱柱（4．6mm×250mm，5μm），以甲醇：0．3％H3PO4=30：70（V/V）为流动相，流速：1．0ml／min，柱温：30℃，检测波长：270nm。[结果]龙胆苦苷在0．9—2．1μg范围内呈良好的线性关系，r=0．9991，平均回收率为97．6％，RSD值为1．02％。[结论]动态热回流提取法的提取效率最高。[Objective] The research aimed to study the effects of different extraction methods on the extraction rate of gentiopicroside by establishing RP- HPLC method and provide theoretical foundation for utilization of C, entiana strominea Maxim. [ Method] Experiments were performed on C18（4.6 mm × 250 mm,5μm） colunm, with CH3OH：0.3% H3PO4（30：70） as mobile phase at a flow rate of 1.0 ml/min. The column tempemlure was 30 ℃ and the determination wavelength was 270 nm. [ Result ] The linear range was 0. 9 - 2.1 μg （ r = 0.999 1 ） and the average recovery was 97.6% （ RSD = 1.02 % ）. [Conclusion] The extraction of gentiopicroside from Gentiana straminea Maxim by reflux with Magnetic Stir was the most efficient.

关 键 词：秦艽 提取方法 龙胆苦苷 提取率 

分 类 号：S567.239[农业科学—中草药栽培]