Th1/Th2细胞因子对器官移植排斥反应的影响  

Impact of Th1/Th2 cytokines on transplant rejection

作　　者：姜晓峰[1] 孙文郁[1] 郭大伟[1] 张弘彬[1] 金俊哲[1] 郑元[1] 赵宇[1] 姜洪磊[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院普外科,辽宁沈阳110032

出　　处：《中国现代普通外科进展》2009年第5期391-393,416,共4页Chinese Journal of Current Advances in General Surgery

基　　金：教育部留学回国人员科研启动基金资助项目(2008-890)

摘　　要：目的:研究Th1/Th2细胞因子对同种异系小鼠心脏移植存活时间的影响。方法:使用野生型BALB/c小鼠作为供体,野生型B6小鼠、IL-4基因去除B6小鼠及IFN-γ基因去除B6小鼠作为受体,行腹部异位小鼠心脏移植。部分IL-4基因去除小鼠、IFN-γ基因去除小鼠联合应用α-半乳糖神经酰胺(α-galactosylceramide,α-GalCer),以获得更强的Th1/Th2偏移。比较移植物存活时间。向野生型、IL-4基因去除及IFN-γ基因去除B6小鼠腹腔内注射供体小鼠脾细胞,提取受体小鼠脾脏CD8+T细胞行淋巴细胞毒试验。结果:IFN-γ基因去除组小鼠的移植物存活时间为(6.40±0.55)d,联合应用α-GalCer组移植物存活时间为(8.00±1.15)d。IL-4基因去除组小鼠的移植物存活时间为(8.00±1.00)d,联合应用α-GalCer组移植物存活时间为(8.60±1.34)d。淋巴细胞毒试验显示IFN-γ基因去除小鼠的CD8+T细胞毒性明显增强。结论:Thl/Th2细胞因子与排斥反应之间并不存在简单的对应关系。Objective： To determine the impact of Th1/Th2 cytokines on transplant rejection. Methods： Intra-abdominal heterotopic heart transplantation was performed using WT BALB/c mice as donors and WT IL-4 KO （knockout） or IFN- γ KO （knockout） B6 mice as recipients. And stronger Th1 or Th2 bias was enabled using a -GalCer and IL-4 KO or tFN- 7 KO mice. For immunization of WT, IL-4 KO or IFN- y KO B6 mice, 3 × 10^7 Mitomycin C （MMC）-treated BALB/c Splenocytes were administered via intraperitoneal injection on day -14 and -7 before the spleens were harvested. The CD8＋T cells were prepared and cytotoxic activity in vitro was assessed by a standard 51Cr release assay. Graft survival times for BALB/c to IFN- γ KO B6 grafts were 6.40 ± 0.55d, and 8.00 ± 1.15 d α -GalCer was combined. Grafts in IL-4 KO recipients rejected their allografts by day 8.00 ± 100,in the group of combination with α -GalCer grafts survived 8.60 ± 1.34 d. 51Cr release assay showed that allospecific CTL activity＇ was increased in IFN-γ KO mice. Conclusions There seems to be no simple relationship between Th1/Th2 cytokines and transplant rejection.

关 键 词：TH1细胞 TH2细胞 细胞因子类 移植物排斥 心脏移植 移植 异位 小鼠 

分 类 号：R617[医药卫生—外科学]