猪生殖与呼吸综合征病毒NX／ZhW／07株的分离鉴定及其ORF5、Nsp2基因特性分析  

Isolation and identification of PRRSV NX/ZhW/07 strain and analysis of characteristics of Nsp2 and ORF5 genes of the strain

作　　者：满自萍[1,2] 田宏[1] 吴锦艳[1] 赵娜[1,2] 尚佑军[1] 杨春生[2] 何生虎[2] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [2]宁夏大学农学院,宁夏银川750021

出　　处：《中国兽医科学》2009年第6期498-502,共5页Chinese Veterinary Science

基　　金：国家“十一五”高技术研究发展计划(863)项目(2006AA10A207)

摘　　要：将2007年宁夏送检的疑似猪高热病病料处理后接种于Marc-145细胞,结果Marc-145细胞出现典型的细胞病变,应用猪生殖与呼吸综合征(PRRS)病原检测试剂盒从细胞培养物中检测到PRRSV,将其命名为NX/ZhW/07。采用RT-PCR方法扩增该分离株的ORF5和Nsp2基因,并进行序列分析。结果表明,NX/ZhW/07株的ORF5基因与GXHP-5、Hainan-1、HUB1、Hunan-1、JIANGSU、Jiangxi-1、LN-5和SHH-5株的核苷酸序列的同源性分别为92%～94%,其中与CH-1a株的核苷酸序列的同源性最高,达99.2%。Nsp2基因序列分析显示,出现30个氨基酸的不连续缺失,与国内高热病分离株JXA1的缺失位置完全一致。由此推测NX/ZhW/07株属于PRRSV美洲型变异株。The samples were collected from swine suspected of having high fever disease in Ningxia in 2007,treated and inoculated to Marc-145 cells, then the typical cytopathogenic effect（CPE） of porcine reproductive and respiratory syndrome virus（PRRSV） was observed and the PRRSV was detected from the cell cultures using the PRRS etiological agent detective Kit and named NX/ZhW/07 strain. The Nsp2 and ORF5 genes of NX/ZhW/07 strain were amplified by RT-PCR and sequenced. The ORF5 gene of NX/ ZhW/07 strain shared 92. 0% -- 94. 0% homology with that of GXHP-5, Hainan-1, HUB1, Hunan-1, JIANGSU,Jiangxi-1,LN-5 and SHH-5, respectively. Between NX/ZhW/07 and CH-la strains,nucleotide homology of ORF5 gene was up to 99.2 %. The deletions of 30 amino acids in NX/ZhW/07 strain was consistent with the highly virulent PRRSV JXA1 strain. The results showed that the strain NX/ZhW/07 might be the mutant isolate of PRRSV American genotype.

关 键 词：猪生殖与呼吸综合征病毒 NX/Zhw/07株 ORF5基因 NSP2基因 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]