利用16S rDNA序列对两种芽孢杆菌的鉴定  

Identification of two species of Bacillus using 16 S ribosomal DNA sequence

作　　者：潘康成[1] 冯兴[1] 崔恒敏[1] 张亚兰[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2009年第6期550-554,共5页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队资助项目(IRT0848);四川省重点建设学科项目(SZD0418)

摘　　要：根据不同种属细菌的16 S rDNA序列两端的保守性区域设计通用引物,提取菌株的基因组DNA,对菌株的16 S rDNA进行了PCR扩增,对扩增到的目标片段进行了测序,将测序结果与NCBI上已知菌种的16 S rDNA序列进行了BLAST对比,并构建了系统进化树。结合传统的形态观察及生理生化特性鉴定,16 S rDNA序列分析结果证实芽孢杆菌Pab02为枯草芽孢杆菌,PAS38为蜡样芽孢杆菌。The conserved primers were designed according to conservative sequence of 16 S rDNA of Bacillus sp. Pab02 and PAS38. The genomic DNAs were extracted from two Bacillus species,then the target 16 S rDNA fragments were amplified by PCR,and the sequence analysis was performed using the NCBI with BLAST data base. According to the morphological and physioehemical properties, and based on their positions in the phylogenetic tree,Pab02 was finally identified to be Bacillus subtilis and PAS38 represented Bacillus cereus.

关 键 词：枯草芽孢杆菌 蜡样芽孢杆菌 16 S RDNA 系统发育分析 鉴定 

分 类 号：S852.616[农业科学—基础兽医学]