曲古抑菌素A对核移植供体细胞组蛋白H3乙酰化水平的影响  

The Effect of Trichostatin A upon Histone H3 Acetylation in Nuclear Transplantation Donor Cells

作　　者：周燕华[1] 贾秀芬[1] 单智焱[1] 郭铁云[1] 刘慧雯[1] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨150081

出　　处：《解剖科学进展》2009年第2期134-137,共4页Progress of Anatomical Sciences

基　　金：黑龙江省科技厅基金(LC02C13);教育部留学归国基金;哈尔滨市科委基金(2002AA9CS151-0-5)

摘　　要：目的探讨组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)对组蛋白H3乙酰化水平的影响,进而提高他们重编程的能力。方法用不同浓度的TSA处理胎儿成纤维细胞,应用免疫荧光和WesternBlot方法对细胞乙酰化水平进行量化分析。结果0.5nM,5nM,50nM和100nM浓度的TSA均引起供体成纤维细胞形态学的变化,对供体成纤维细胞组蛋白H3乙酰化有明显的剂量效应,随着TSA浓度增加,组蛋白H3乙酰化水平逐渐增加,在TSA浓度5nM时比0.5nM增加尤为明显。结论TSA能够提高胎儿成纤维细胞组蛋白H3乙酰化水平,提高核移植重编程的能力。Objective To study the effect of trichostatin A （ TSA ） upon histone H3 acetylation in nuclear transplantation （ NT ） donor cells, and improve their reprogramming. Method Mouse embryonic fibroblasts （ MEF ） were cultured and treated by different concentrations of TSA and MEF histone H3 acetylation was analysed using immunofluorescence and Western blot. Result TSA can significantly improve the histonc H3 acetylation in donor fibroblasts, the level of histone H3 acetylation increased as the increase of TSA concentrations, TSA with the different concentrations of 0.5UM, 5nM, 50nM and 100nM can alter donor cell morphology. Conclusion TSA promotes histone H3 acetylation and NT reprogramming capacity in MEF.

关 键 词：曲古抑菌素A 胎儿成纤维细胞 组蛋白乙酰化 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]